韓國建國大學(xué)一研究團(tuán)隊(duì)日前成功地從人體傷口周邊組織中提取到皮膚干細(xì)胞，此項(xiàng)成果有望推動(dòng)干細(xì)胞研究加速發(fā)展,。
此間媒體報(bào)道說,，在一項(xiàng)共同研究中，建國大學(xué)的研究人員從剖腹產(chǎn)手術(shù)時(shí)產(chǎn)生的手術(shù)傷口組織中提取到干細(xì)胞,，通過骨形態(tài)發(fā)生蛋白（bmp-4）增殖分化,，成功獲得了大量干細(xì)胞。
實(shí)驗(yàn)證明,，使用該方法獲得的干細(xì)胞能夠成功被誘導(dǎo)分化為神經(jīng)細(xì)胞,。這些神經(jīng)細(xì)胞將被用于自體干細(xì)胞移植和干細(xì)胞醫(yī)療等領(lǐng)域的后續(xù)實(shí)驗(yàn),。研究人員相信，提取自傷口組織的人體干細(xì)胞的分化能力同胚胎干細(xì)胞相當(dāng),。
研究人員表示,，將上述皮膚干細(xì)胞誘導(dǎo)分化為胰島素分泌細(xì)胞的實(shí)驗(yàn)現(xiàn)已取得階段性成果，下一步,，他們將探索通過細(xì)胞移植治療糖尿病的可行性,。
此前，科學(xué)家已經(jīng)在人體皮膚細(xì)胞中獲得了成體干細(xì)胞,，但提取效率低下,，無法獲得研究工作所需要的最低數(shù)量。
評論認(rèn)為,，此項(xiàng)即將發(fā)表在美國《組織工程學(xué)》雜志上的研究成果受到了學(xué)術(shù)界的廣泛關(guān)注,。使用胚胎干細(xì)胞進(jìn)行克隆研究已引發(fā)大量倫理問題，胚胎干細(xì)胞還存在可獲得性問題,。從傷口組織提取干細(xì)胞的方法讓大量獲取皮膚干細(xì)胞成為可能,，有望開啟干細(xì)胞研究的新局面,。（生物谷Bioon.com）
生物谷推薦原始出處：
Tissue Engineering Part C: Methods September 18, 2009 doi:10.1089/ten.TEC.2009.0275.
Skin-derived Stem Cells in Human Scar Tissues: A Novel Isolation and Proliferation Technique and Their Differentiation Potential to Neurogenic Progenitor Cells
Mr. Ji Hoon Yang Dr. Sang Woo Shim Prof. Bo Yon Lee Dr. Hoon Taek Lee, Prof
Adult tissues contain stem cells that can transdifferentiate into other cell lineages besides forming differentiated cells of their own tissue of origin. However, human adult skin-derived stem cells have a very low efficiency. Here, we established a novel culture system involving BMP-4 and a floating culture system with sphere producing medium (SPM) that can enrich adult stem cell populations in vitro. Adult stem cells were isolated from useless human scar tissue. Like mesenchymal stem cells, cultured human scar tissue-derived stem cells (hSTSCs) altered their morphology and significantly increased the number of Nestin-positive cells in proportion to the alkaline phosphatase-positive cell ratio. Moreover, the expression of the pluripotency regulator Oct-4 and its target transcripts, Sox-2, c-kit, and Rex-1, was also stimulated by this culture system. Differentiation of neurogenic progenitor cells using basic fibroblast growth factor (bFGF) and Neurogen 2 was successfully performed in vitro more rapidly than previous reports. Differentiation potential into three germ layer was also performed that our hSTSCs possessed differentiation potential into various cell type both cell and sphere. Neuronal differentiation results that our hSTSCs expressed marker of neurogenic genes, such as glial fibrillary acid protein, neural cell adhesion molecules (NCAM), neuron filament-M, and microtubule-associated protein 2. These results suggest that BMP-4 and the floating culture system with SPM induced significant proliferation of hSTSCs and mediated reprogramming of the cells from adult somatic tissue into precursor state to some degree. It is thought that this new culture system might be a simple, effective, and easily manageable process for regenerative tissue repair and autotransplantation.
