8月6日,，美國和日本的研究人員在《細胞》（Cell）雜志網絡版上發(fā)表論文稱,，他們通過在成纖維原細胞中植入特定的Gata4, Mef2c和Tbx5種基因，成功培育出心肌細胞,。研究人員發(fā)現(xiàn)，在小鼠胚胎的心臟中，有3種基因是生成心肌細胞必不可少的,。通過向纖維原細胞中植入這3種基因，可以獲得驅動心跳的心肌細胞,。與利用誘導多能干細胞(iPS細胞)培育心肌細胞相比,，這種方法更加安全、簡捷,。
據(jù)新華社記者采訪該項研究負責人家田真樹,這位日本慶應大學助教說：“今后將確認是否可以用同樣方法制造出人類心肌細胞,。如果可行，心肌梗塞患者將無需接受開胸手術,，而只需通過導入這些基因,，讓那里的纖維原細胞直接生成健康的心肌細胞。” （生物谷Bioon.com）
日本成功用心臟干細胞實現(xiàn)心肌再生
骨髓間充質干細胞體內誘導分化為心肌細胞
楊黃恬 - 2010年干細胞技術與應用講座
2008-2010年國家自然科學基金資助干細胞項目
CIRCULATION：心肌再生研究取得重要進展
生物谷推薦原文出處：
Cell doi：10.1016/j.cell.2010.07.002
Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes by Defined Factors
Masaki Ieda, Ji-Dong Fu, Paul Delgado-Olguin, Vasanth Vedantham, Yohei Hayashi, Benoit G. Bruneau, Deepak Srivastava
The reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs) raises the possibility that a somatic cell could be reprogrammed to an alternative differentiated fate without first becoming a stem/progenitor cell. A large pool of fibroblasts exists in the postnatal heart, yet no single “master regulator” of direct cardiac reprogramming has been identified. Here, we report that a combination of three developmental transcription factors (i.e., Gata4, Mef2c, and Tbx5) rapidly and efficiently reprogrammed postnatal cardiac or dermal fibroblasts directly into differentiated cardiomyocyte-like cells. Induced cardiomyocytes expressed cardiac-specific markers, had a global gene expression profile similar to cardiomyocytes, and contracted spontaneously. Fibroblasts transplanted into mouse hearts one day after transduction of the three factors also differentiated into cardiomyocyte-like cells. We believe these findings demonstrate that functional cardiomyocytes can be directly reprogrammed from differentiated somatic cells by defined factors. Reprogramming of endogenous or explanted fibroblasts might provide a source of cardiomyocytes for regenerative approaches.
