生物谷Bioon.com 訊 中國臺(tái)灣一家科研機(jī)構(gòu)研究團(tuán)隊(duì)利用胎兒臍帶血管里的人類臍靜脈內(nèi)皮細(xì)胞,，并加入 2個(gè)非致癌性基因,，成功將內(nèi)皮細(xì)胞轉(zhuǎn)變成萬能干細(xì)胞，且免除有致癌風(fēng)險(xiǎn)基因,，研究成果和國際干細(xì)胞研究同步,。
臺(tái)灣衛(wèi)生研究院細(xì)胞及系統(tǒng)醫(yī)學(xué)研究所副研究員顏伶汝今天表示，2006年和2007年日本和美國分別宣布發(fā)現(xiàn)將普通皮膚細(xì)胞轉(zhuǎn)化為干細(xì)胞的方法,，這種干細(xì)胞功能與胚胎干細(xì)胞相差不多,，因此被稱為萬能干細(xì)胞，簡(jiǎn)稱iPS細(xì)胞,。
不過,，顏伶汝說，以日本的研究來說,，必須加入 4個(gè)基因，才能把皮膚的纖維母細(xì)胞誘導(dǎo)成與胚胎干細(xì)胞類似的干細(xì)胞,；但是,，在4個(gè)基因中，包括c-MYC及KLF4卻有讓細(xì)胞癌化的能力,，因此運(yùn)用在臨床上仍有疑慮,。
為了避免加入致癌性基因、又可以成功發(fā)展出萬能干細(xì)胞,，顏伶汝說,，“國衛(wèi)院”團(tuán)隊(duì)利用胎兒臍帶血管里的人體臍靜脈內(nèi)皮細(xì)胞HUVEC,，只需要利用2個(gè)非致癌性基因OCT4及SOX2，就可以把HUVEC內(nèi)皮細(xì)胞轉(zhuǎn)變成萬能干細(xì)胞,，也不會(huì)讓細(xì)胞癌化,。
她說，因?yàn)镠UVEC內(nèi)皮細(xì)胞取自新生兒臍帶,，具有取得容易的優(yōu)點(diǎn),，且能夠成功避免有致癌疑慮的基因，這項(xiàng)研究也是臺(tái)灣在這項(xiàng)領(lǐng)域第一個(gè)杰出的成就,，并且能和國際的干細(xì)胞研究同步,。
她說，這個(gè)萬能干細(xì)胞也增加臨床應(yīng)用的機(jī)會(huì),，像是可能發(fā)展出神經(jīng)細(xì)胞,、肝臟細(xì)胞等，甚至運(yùn)用在藥物測(cè)試,。（生物谷Bioon.com）
生物谷推薦原文出處：
Arteriosclerosis, Thrombosis, and Vascular Biology doi: 10.1161/ATVBAHA.110.206540
Endogenous KLF4 Expression in Human Fetal Endothelial Cells Allows for Reprogramming to Pluripotency With Just OCT3/4 and SOX2
Pai-Jiun Ho ; Men-Luh Yen ; Jhong-De Lin ; Lan-Sun Chen ; Hsin-I Hu ; Chun-Kai Yeh ; Chiu-Ying Peng ; Chen-Yu Lin ; Shaw-Fang Yet ; and B. Linju Yen *
The introduction of 4 transcription factors—c-MYC, OCT3/4, SOX2, and KLF4—can reprogram somatic cells back to pluripotency. However, some of the factors used are oncogenic, making therapeutic application unfeasible. Although the use of adult stem cells expressing high endogenous levels of some of these factors allows for reprogramming with fewer exogenous genes, such cells are rare and may have accumulated genetic mutations. Our goal was to reprogram human somatic cells without oncogenic factors. We found that high endogenous expression of KLF4 in human umbilical vein endothelial cells (HUVECs) allows for generation of induced pluripotent stem cells (iPSCs) with just 2 nononcogenic factors, OCT3/4 and SOX2. HUVECs were infected with lentivirus containing OCT4 and SOX2 for generation of iPSCs. These 2-factor HUVEC iPSCs were morphologically similar to embryonic stem cells, express endogenous pluripotency markers postreprogramming, and can differentiate toward lineages of all 3 germ layers both in vitro and in vivo. iPSCs can be generated from HUVECs with only 2 nononcogenic factors. The use of fetal cells for reprogramming without oncogenic factors may provide an efficient in vitro model for human iPSC research, as well as a novel source for possible therapeutic use.
