隨著再生醫(yī)學的發(fā)展,，干細胞治療逐步成為一些臨床重要疾病的治療手段之一,。人體內(nèi)存在著一定數(shù)量的組織干細胞或前體細胞，它們具有多向分化潛能,，是參與組織再生和創(chuàng)傷修復的種子細胞,。對于干細胞治療而言，利用自體干細胞修復組織損傷是最安全的,。但是如何有效利用自體組織干細胞參與組織再生和創(chuàng)傷修復,？
中國科學院遺傳與發(fā)育生物學所戴建武研究員領(lǐng)導的再生醫(yī)學實驗室研制出了能捕捉組織干細胞的膠原生物材料，并與南京市鼓樓醫(yī)院心血管外科合作,，在動物模型上驗證了該材料可以有效富集組織干細胞并引導組織再生,。
他們制備了小鼠心肌損傷模型，用能捕捉組織干細胞的膠原材料修補缺損的心肌組織,，發(fā)現(xiàn)手術(shù)后一個月該材料能有效捕捉和富集組織干細胞,，三個月時該材料可以有效促進心肌組織的再生,。戴建武研究員認為，利用自體干細胞修復組織損傷是最安全的干細胞應用方式,，該材料不僅適合于心肌組織損傷修復,，同樣適用于皮膚、神經(jīng),、骨等組織的再生與損傷修復,。
這項工作以戴建武實驗室博士研究生施春英和南京市鼓樓醫(yī)院心血管外科李慶國為共同第一作者，發(fā)表在Biomaterials（doi:10.1016/j.biomaterials.2010.12.026）上,。（生物谷Bioon.com）
生物谷推薦原文出處：
Biomaterials doi:10.1016/j.biomaterials.2010.12.026
Stem-cell-capturing collagen scaffold promotes cardiac tissue regeneration
Chunying Shi,Qingguo Li,Yannan Zhao,Wei Chen,Bing Chen,Zhifeng Xiao,Hang Lin,Ling Nie,Dongjin Wang,and Jianwu Dai
Abstract
Stem cell based therapy is coming of age. Besides stem cell transplantation, it has been a goal to use native autologous stem cells for tissue regeneration. However, the recruitment of native autologous stem cells at the targeting site has not been sufficient which limits the clinical application of autologous stem cells. Biomaterials have been increasingly used in tissue repair. They not only serve as scaffolds for cell proliferation, differentiation, and also provide guidance for 3-D reestablishment. In this study, we have attempted to enrich autologous stem cells at the wound site through a stem-cell-capturing collagen scaffold by conjugating with a stem cell specific antibody. Sca-1 is a common surface marker of hematopoietic, cardiac and skeletal muscle stem cells. Due to the interaction of antibody and antigen, Sca-1 positive cells could be enriched to the functional collagen scaffold both in vitro and in vivo. When the functional collagen scaffold is transplanted into C57/BL6 mouse as a patch to repair a surgical heart defect, the regeneration of cardiomyocytes has been observed. Thus, the collagen scaffolds covalently conjugated with stem cell specific antibody could be an effective approach to promote tissue regeneration.
