日本研究人員開發(fā)出了能測量細(xì)胞間黏合力的技術(shù),，利用這一技術(shù)將有助于了解癌細(xì)胞的轉(zhuǎn)移機制，檢測人工培育的組織細(xì)胞是否正常黏合在一起,。
奈良尖端科學(xué)技術(shù)研究生院和近畿大學(xué)的研究小組,，日前在新一期美國《國家科學(xué)院學(xué)報》網(wǎng)絡(luò)版上報告說，由于細(xì)胞極其微小,、脆弱且緊密黏合在一起,，所以將細(xì)胞分離開并測量它們之間的黏合力一直非常困難。
研究人員利用脈沖極短,、瞬時功率超高的飛秒激光照射有細(xì)胞的培養(yǎng)液,，產(chǎn)生沖擊波,，然后利用能夠發(fā)現(xiàn)細(xì)微結(jié)構(gòu)的原子力顯微鏡，觀測沖擊波并換算成力,。通過變換激光的強度,，測試多大強度的沖擊波能夠?qū)⒓?xì)胞分離開，就可以測量出細(xì)胞間的黏合力有多大,。
報告指出,，有研究者認(rèn)為，癌細(xì)胞是通過緊密附著在血管內(nèi)側(cè)的細(xì)胞上而轉(zhuǎn)移的,，因此這一技術(shù)將有助于了解癌細(xì)胞的轉(zhuǎn)移機制,，此外還可用該技術(shù)檢測以干細(xì)胞培育出的組織細(xì)胞是否正常黏合在一起。（生物谷Bioon.com）
生物谷推薦原文出處：
PNAS doi: 10.1073/pnas.1006847108
Noncontact estimation of intercellular breaking force using a femtosecond laser impulse quantified by atomic force microscopy
Yoichiroh Hosokawaa,1, Man Hagiyamab, Takanori Iinoa, Yoshinori Murakamib, and Akihiko Itoc,1
Abstract
When a femtosecond laser pulse (fsLP) is focused through an objective lens into a culture medium, an impulsive force (fsLP-IF) is generated that propagates from the laser focal point (Of) in a micron-sized space. This force can detach individual adherent cells without causing considerable cell damage. In this study, an fsLP-IF was reflected in the vibratory movement of an atomic force microscopy (AFM) cantilever. Based on the magnitude of the vibration and the geometrical relationship between Of and the cantilever, the fsLP-IF generated at Of was calculated as a unit of impulse [N-s]. This impulsive force broke adhesion molecule-mediated intercellular interactions in a manner that depended on the adhesion strength that was estimated by the cell aggregation assay. The force also broke the interactions between streptavidin-coated microspheres and a biotin-coated substrate with a measurement error of approximately 7%. These results suggest that fsLP-IF can be used to break intermolecular and intercellular interactions and estimate the adhesion strength. The fsLP-IF was used to break intercellular contacts in two biologically relevant cultures: a coculture of leukocytes seeded over on an endothelial cell monolayer, and a polarized monolayer culture of epithelial cells. The impulses needed to break leukocyte–endothelial and interepithelial interactions, which were calculated based on the geometrical relationship between Of and the adhesive interface, were on the order of 10-13 and 10-12 N-s, respectively. When the total impulse at Of is well-defined, fsLP-IF can be used to estimate the force required to break intercellular adhesions in a noncontact manner under biologically relevant conditions.
