近日英國出版的《分子膜生物學(xué)》(Molecular Membrane Biology)雜志刊登了我所王鑫研究員等完成的關(guān)于化石植物中膜融合過程的研究論文,。這項(xiàng)研究展示了植物細(xì)胞中難得一見的膜融合瞬間的膜結(jié)構(gòu)。
膜融合與細(xì)胞中的很多生理活動(dòng)過程有密切的關(guān)系,。由于這個(gè)過程轉(zhuǎn)瞬即逝,,在現(xiàn)代結(jié)構(gòu)生物學(xué)研究中一直是一個(gè)難于捕捉和研究的關(guān)鍵問題之一。此前人們?cè)?jīng)報(bào)道過,,膜融合過程中形成的融合孔的中央會(huì)形成一個(gè)中央塞,,但是這個(gè)中央塞的來源卻知之不詳,。在古生物學(xué)的傳統(tǒng)觀念中,化石中與生命活動(dòng)密切相關(guān)的細(xì)胞質(zhì)被認(rèn)為完全降解了,,因此古植物的生理活動(dòng)被認(rèn)為是一個(gè)無從談起的研究目標(biāo),。但是近來的古植物學(xué)研究發(fā)現(xiàn)了越來越多的植物細(xì)胞質(zhì)化石及其超微結(jié)構(gòu)。此前王鑫團(tuán)隊(duì)曾于2007年報(bào)道了植物化石細(xì)胞中的膜融合瞬間,,但是并不知道這個(gè)過程的詳細(xì)步驟,。
在新發(fā)表的論文中,王鑫等人在前面研究的基礎(chǔ)上進(jìn)一步深入研究了美國中新世植物化石中的膜融合現(xiàn)象,。經(jīng)過長時(shí)間仔細(xì)的觀察,,他們發(fā)現(xiàn)了融合孔中形成中央塞的過程:融合孔中殘余的小泡膜和細(xì)胞膜共同形成了中央塞,其中小泡膜和細(xì)胞膜的內(nèi)層形成一個(gè)恨水基在外的盤狀結(jié)構(gòu),。這是人們首次觀察到即使在現(xiàn)代生物細(xì)胞中都很難發(fā)現(xiàn)的細(xì)節(jié),。它在化石中的出現(xiàn)為人們解讀膜融合的過程提供了珍貴的線索,也為模擬膜融合過程提供了獨(dú)特的參考依據(jù),。這個(gè)難得證據(jù)也暗示固定這一瞬間的過程是迅速完成的,,很可能與雷電現(xiàn)象有關(guān)系,。(生物谷Bioon.com)
生物谷推薦原文出處:
Molecular Membrane Biology doi:10.3109/09687688.2010.536169
Palaeontological evidence of membrane relationship in step-by-step membrane fusion
Xin Wang 1, Wenzhe Liu2, & Kaihe Du3
Studies on membrane fusion in living cells indicate that initiation of membrane fusion is a transient and hard to capture process. Despite previous research, membrane behaviour at this point is still poorly understood. Recent palaeobotanical research has revealed snapshots of membrane fusion in a 15-million-year-old fossil pinaceous cone. To reveal the membrane behaviour during the fusion, we conducted more observations on the same fossil material. Several discernible steps of membrane fusion have been fixed naturally and observed in the fossil material. This observation provides transmission electron microscope (TEM) images of the transient intermediate stage and clearly shows the relationship between membranes. Observing such a transient phenomenon in fossil material implies that the fixing was most likely accomplished quickly by a natural process. The mechanism behind this phenomenon is clearly worthy of further enquiry.
Keywords: Membrane, relationship, fusion, quick, fixing, lightning