科學(xué)家在日前出版的《自然—化學(xué)生物學(xué)》Nature Chemical Biology上撰文稱,他們發(fā)現(xiàn)了合成角質(zhì)素所需要的酶,,角質(zhì)素是一種存在于植物表皮中的聚合物,。這項(xiàng)發(fā)現(xiàn)將給植物研究帶來影響并為農(nóng)業(yè)提供幫助,。
植物的角質(zhì)層,或稱為表皮,,有保持植物自身水分的作用,,同時也能保護(hù)植物不受外界侵襲。角質(zhì)層的主要成分就是角質(zhì)素,,但是人們一直都不了解角質(zhì)素是如何生成的,。先前推測認(rèn)為,GDSLs這一酶家族可能與角質(zhì)素合成有關(guān),,但GDSLs酶家族的作用功能眾多,,要想從中鑒定出特定的酶也變得具有挑戰(zhàn)性。
Jocelyn Rose等人利用西紅柿中具有角質(zhì)素合成缺陷的一種突變種類來找尋這種神秘的酶,,最終發(fā)現(xiàn)該酶其實(shí)屬于GDSLs酶家族中的一種,。缺少這種酶會導(dǎo)致底物的積累,實(shí)驗(yàn)結(jié)果也顯示這種酶可合成帶有預(yù)期功能的短鏈酯分子,。(生物谷Bioon.com)
doi:10.1038/nchembio.960
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The identification of cutin synthase: formation of the plant polyester cutin
Trevor H Yeats, Laetitia B B Martin, Hélène M-F Viart, Tal Isaacson, Yonghua He, Lingxia Zhao, Antonio J Matas, Gregory J Buda, David S Domozych, Mads H Clausen & Jocelyn K C Rose
A hydrophobic cuticle consisting of waxes and the polyester cutin covers the aerial epidermis of all land plants, providing essential protection from desiccation and other stresses. We have determined the enzymatic basis of cutin polymerization through characterization of a tomato extracellular acyltransferase, CD1, and its substrate, 2-mono(10,16-dihydroxyhexadecanoyl)glycerol. CD1 has in vitro polyester synthesis activity and is required for cutin accumulation in vivo, indicating that it is a cutin synthase.