在干細胞生物學(xué)中,，如何保存從健康人或者患者身上獲得的干細胞并維持其多能性是一個巨大的挑戰(zhàn),。本文中，研究人員發(fā)現(xiàn),，在死后17天的人和死后14天的小鼠骨骼肌中分離的干細胞依然能夠存活并維持其多能性,，這一時間比之前的報道要長得多,。
在尸體中，肌肉干細胞能在組織中形成富集,，這說明與其它細胞相比，肌肉干細胞存在著明顯的生存優(yōu)勢,。研究人員發(fā)現(xiàn),，這些細胞能夠進行一種可逆的休眠，其顯著標志是代謝活動減慢,、第一次分裂時間的滯后、活性氧簇水平升高和預(yù)活化狀態(tài)的轉(zhuǎn)錄子減少,。這種休眠對于細胞保持生存能力是至關(guān)重要的,。
研究人員稱，這些細胞為干細胞生物學(xué)研究提供了有用資源,。（生物谷 Bioon.com ）
doi:10.1038/ncomms1890
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Skeletal muscle stem cells adopt a dormant cell state post mortem and retain regenerative capacity
Mathilde Latil, Pierre Rocheteau, Laurent Châtre, Serena Sanulli, Sylvie Mémet, Miria Ricchetti, Shahragim Tajbakhsh & Fabrice Chrétien
The accessibility to stem cells from healthy or diseased individuals, and the maintenance of their potency are challenging issues for stem cell biology. Here we report the isolation of viable and functional skeletal myogenic cells from humans up to 17 days, and mice up to 14 days post mortem, much longer beyond previous reports. Muscle stem cells are enriched in post mortem tissue, suggesting a selective survival advantage compared with other cell types. Transplantation of mouse muscle and haematopoietic stem cells regenerates tissues robustly. Cellular quiescence contributes to this cell viability where cells adopt a reversible dormant state characterized by reduced metabolic activity, a prolonged lag phase before the first cell division, elevated levels of reactive oxygen species and a transcriptional status less primed for commitment. Finally, severe hypoxia, or anoxia is critical for maintaining stem cell viability and regenerative capacity. Thus, these cells provide a useful resource for studying stem cell biology.
