核小體在基因組DNA上的位置會(huì)影響染色體功能的很多方面。近日刊登在國際著名雜志Nature上的一篇研究報(bào)道揭示了釀酒酵母核小體分布圖,。
Kristin Brogaard等人建立了一種新穎的方法,，來在全基因組范圍內(nèi)對(duì)釀酒酵母的核小體位置進(jìn)行活體分析，其辦法是利用高度局部化的羥基自由基來修飾工程改造的組蛋白。
所獲得的分布圖以單堿基對(duì)的準(zhǔn)確性限定了核小體中心的位置。核小體的精確位置被發(fā)現(xiàn)與各種不同的基因組特征相關(guān)，其中包括轉(zhuǎn)位子集成點(diǎn)的精確位置以及RNA聚合酶的停頓點(diǎn),。（生物谷Bioon.com）
doi:10.1038/nature11142
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A map of nucleosome positions in yeast at base-pair resolution
Kristin Brogaard, Liqun Xi, Ji-Ping Wang & Jonathan Widom
The exact positions of nucleosomes along genomic DNA can influence many aspects of chromosome function. However, existing methods for mapping nucleosomes do not provide the necessary single-base-pair accuracy to determine these positions. Here we develop and apply a new approach for direct mapping of nucleosome centres on the basis of chemical modification of engineered histones. The resulting map locates nucleosome positions genome-wide in unprecedented detail and accuracy. It shows new aspects of the in vivo nucleosome organization that are linked to transcription factor binding, RNA polymerase pausing and the higher-order structure of the chromatin fibre.
