紐約州立大學(xué)石溪分校骨科生物工程研究實(shí)驗(yàn)室主任Yi-Xian Qin領(lǐng)導(dǎo)完成的一項(xiàng)研究表明,，中等強(qiáng)度的超聲能刺激成骨細(xì)胞的流動(dòng)性,，并觸發(fā)其鈣的釋放，鈣釋放促骨細(xì)胞的增長,。該技術(shù)可以提供一種方法來開發(fā)非藥物治療骨質(zhì)疏松癥,、骨折等涉及骨質(zhì)流失癥狀的疾病。
像骨骼,、肌肉等組織受到機(jī)械負(fù)荷和應(yīng)激如運(yùn)動(dòng)等刺激后存在強(qiáng)大的動(dòng)態(tài)平衡狀態(tài),。Qin醫(yī)生和石溪分校的同事Shu Zhang,、Jiqi Cheng正研究成骨細(xì)胞是如何應(yīng)對機(jī)械信號如超聲的,。
在小鼠細(xì)胞實(shí)驗(yàn)?zāi)Ｐ椭校芯啃〗M創(chuàng)造了一種新的聲輻射力（ARF）的超聲形式,，只適用于一分鐘內(nèi)單個(gè)成骨細(xì)胞,。他們一致認(rèn)為ARF通過聚焦超聲束誘導(dǎo)細(xì)胞骨架重排，促進(jìn)細(xì)胞的活力和流動(dòng),，加速細(xì)胞內(nèi)鈣離子運(yùn)輸和濃度,。
Qin醫(yī)生表示該裝置正在開發(fā)作為一種新的診斷工具，可以預(yù)測早期骨丟失,。該項(xiàng)研究項(xiàng)目是由國立衛(wèi)生研究院,、國家空間生物醫(yī)學(xué)研究所部分資助。（生物谷：Bioon.com）
doi:10.1371/journal.pone.0038343
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Mechanobiological Modulation of Cytoskeleton and Calcium Influx in Osteoblastic Cells by Short-Term Focused Acoustic Radiation Force
Shu Zhang1,2, Jiqi Cheng1, Yi-Xian Qin1*
Mechanotransduction has demonstrated potential for regulating tissue adaptation in vivo and cellular activities in vitro. It is well documented that ultrasound can produce a wide variety of biological effects in biological systems. For example, pulsed ultrasound can be used to noninvasively accelerate the rate of bone fracture healing. Although a wide range of studies has been performed, mechanism for this therapeutic effect on bone healing is currently unknown. To elucidate the mechanism of cellular response to mechanical stimuli induced by pulsed ultrasound radiation, we developed a method to apply focused acoustic radiation force (ARF) (duration, one minute) on osteoblastic MC3T3-E1 cells and observed cellular responses to ARF using a spinning disk confocal microscope. This study demonstrates that the focused ARF induced F-actin cytoskeletal rearrangement in MC3T3-E1 cells. In addition, these cells showed an increase in intracellular calcium concentration following the application of focused ARF. Furthermore, passive bending movement was noted in primary cilium that were treated with focused ARF. Cell viability was not affected. Application of pulsed ultrasound radiation generated only a minimal temperature rise of 0.1°C, and induced a streaming resulting fluid shear stress of 0.186 dyne/cm2, suggesting that hyperthermia and acoustic streaming might not be the main causes of the observed cell responses. In conclusion, these data provide more insight in the interactions between acoustic mechanical stress and osteoblastic cells. This experimental system could serve as basis for further exploration of the mechanosensing mechanism of osteoblasts triggered by ultrasound.
