在一項最新研究中,，來自美國印第安納大學醫(yī)學院的研究人員比較了來自人胎盤和來自人臍帶血的內皮集落形成細胞(endothelial colony-forming cells, ECFCs)哪個擁有更強的增殖能力和更好地形成新的血管,，結果發(fā)現(xiàn)來自人胎盤的ECFCs更好地產生新血管。相關研究結果發(fā)表在Cell Medicine期刊上,。
研究通信作者Michael P. Murphy博士說,，“從人臍帶血分離出的循環(huán)流通ECFCs(circulating ECFCs)和從人胎盤中分離出的常駐ECFCs(resident ECFCs)在表型上是一樣，而且擁有同樣的增殖潛力,。在移植之后,，胎盤來源的常駐ECFCs要比來自臍帶血中的循環(huán)流通ECFCs產生明顯更加多的血管，這就表明常駐ECFCs和循環(huán)流通ECFCs之間存在內在性的功能差別,，源自胎盤的ECFCs產生更加多的新血管,。”
研究人員說，臍帶血和胎盤胚外膜都是祖細胞的理想來源。然而,，從胎盤中能夠獲得的細胞量要比從臍帶血中獲得的數(shù)量大得多,，這就使得胎盤成為細胞量更為充足的來源。他們作出結論,，胎盤代表著一種ECFCs數(shù)量充足的來源,，能夠提供大量用于治療的細胞。(生物谷：Bioon.com)
本文編譯自Blood vessel forming potential of stem cells from human placenta and umbilical cord blood
doi: 10.3727/215517911X617888
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Resident Endothelial Progenitor Cells From Human Placenta Have Greater Vasculogenic Potential Than Circulating Endothelial Progenitor Cells From Umbilical Cord Blood
Rapp, Brian M.; Saadatzedeh, M. Reza; Ofstein, Richard H.; Bhavsar, Janak R.; Tempel, Zachary S.; Moreno, Oscar; Morone, Peter; Booth, Dana A.; Traktuev, Dmitry O.; Dalsing, Michael C.; Ingram, David A.; Yoder, Mervin C.; March, Keith L.; Murphy, Michael P.
Endothelial colony-forming cells (ECFCs) isolated from umbilical cord blood (CBECFCs) are highly proliferative and form blood vessels in vivo. The purpose of this investigation was to isolate and characterize a population of resident ECFCs from the chorionic villi of term human placenta and provide a comparative analysis of their proliferative and vasculogenic potential with CBECFCs. ECFCs were isolated from umbilical cord blood and chorionic villi from placentas obtained by caesarean deliveries. Placental ECFCs (PECFCs) expressed CD144, CD31, CD105, and KDR and were negative for CD45 and CD34, consistent with other ECFC phenotypes. PECFCs were capable of 28.6 ± 6.0 population doublings before reaching senescence (vs. 47.4 ± 3.2 for CBECFCs, p < 0.05, n = 4). In single cell assays, 46.5 ± 1.2% underwent at least one division (vs. 51.0 ± 1.8% of CBECFCs, p = 0.07, n = 6), and of those dividing PECFCs, 71.8 ± 0.9% gave rise to colonies of >500 cells (highly proliferative potential clones) over 14 days (vs. 69.4 ± 0.7% of CBECFCs, p = 0.07, n = 9). PECFCs formed 5.2 ± 0.8 vessels/mm2 in collagen/fibronectin plugs implanted into nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice, whereas CBECFCs formed only 1.7 ± 1.0 vessels/mm2 (p < 0.05, n = 4). This study demonstrates that circulating CBECFCs and resident PECFCs are identical phenotypically and contain equivalent quantities of high proliferative potential clones. However, PECFCs formed significantly more blood vessels in vivo than CBECFCs, indicating that differences in vasculogenic potential between circulating and resident ECFCs exist.
