2013年6月1日訊/生物谷BIOON/--加拿大Renaud Manuguerra-Gagne等學(xué)者日前公布關(guān)于干細(xì)胞治療青光眼的最新研究成果：在青光眼模型中移植入間充質(zhì)干細(xì)胞,，通過激光誘發(fā)的旁分泌因子分泌和祖細(xì)胞增殖可以促進(jìn)青光眼的組織再生,。該論文發(fā)表在學(xué)術(shù)期刊《干細(xì)胞》5月22日一期的再生醫(yī)學(xué)版塊。
骨髓細(xì)胞中的造血和間充質(zhì)成分有助于受損器官的修復(fù),，因此常用于急性病的治療,，但很少用于慢性病的治療。Renaud等人嘗試用間充質(zhì)干細(xì)胞治療慢性青光眼,。在研究中，他們用激光誘發(fā)了一個(gè)開角型青光眼模型,，去評(píng)估骨髓細(xì)胞的治療潛能和組織修復(fù)的機(jī)制,；然后用激光誘發(fā)的方式將效應(yīng)細(xì)胞導(dǎo)入受損組織，研究激光誘發(fā)的效果,。
實(shí)驗(yàn)顯示：骨髓中的間充質(zhì)干細(xì)胞誘發(fā)小梁網(wǎng)細(xì)胞的再生,，注射進(jìn)眼前房比造血細(xì)胞更能有效引起眼內(nèi)壓(IOP)下降(p<.001)。此外,，間充質(zhì)干細(xì)胞和它們分泌的因子誘發(fā)睫狀體中祖細(xì)胞池再活化,，促進(jìn)細(xì)胞增殖。激光誘發(fā)的組織重構(gòu)將間充質(zhì)干細(xì)胞按照預(yù)定目標(biāo)導(dǎo)入受損區(qū)域，并使眼祖細(xì)胞也有一定的增加,。
因此,，Renaud等人的這項(xiàng)試驗(yàn)可以確定間充質(zhì)干細(xì)胞及其分泌組分是開角型青光眼通過局部神經(jīng)祖細(xì)胞進(jìn)行組織修復(fù)的關(guān)鍵介質(zhì)。此外,，也證實(shí)慢性病的干細(xì)胞治療中,，激光療法代表一個(gè)頗具吸引力的治療策略。（生物谷Bioon.com）
供稿源：漢氏聯(lián)合專家團(tuán)隊(duì)翻譯
生物谷推薦英文摘要：
Stem Cells. 2013 Jun;31(6):1136-48. doi:10.1002/stem.1364
Transplantation of Mesenchymal Stem Cells Promotes Tissue Regeneration in a Glaucoma Model Through Laser-Induced Paracrine Factor Secretion and Progenitor Cell Recruitment
Among bone marrow cells, hematopoietic and mesenchymal components can contribute to repair damaged organs. Such cells are usually used in acute diseases but few options are available for the treatment of chronic disorders. In this study, we have used a laser-induced model of open angle glaucoma (OAG) to evaluate the potential of bone marrow cell populations and the mechanisms involved in tissue repair. In addition, we investigated laser-induced tissue remodeling as a method of targeting effector cells into damaged tissues. We demonstrate that among bone marrow cells, mesenchymal stem cells (MSC) induce trabecular meshwork regeneration. MSC injection into the ocular anterior chamber leads to far more efficient decrease in intraocular pressure (IOP) (p < .001) and healing than hematopoietic cells. This robust effect was attributable to paracrine factors from stressed MSC, as injection of conditioned medium from MSC exposed to low but not to normal oxygen levels resulted in an immediate decrease in IOP. Moreover, MSC and their secreted factors induced reactivation of a progenitor cell pool found in the ciliary body and increased cellular proliferation. Proliferating cells were observed within the chamber angle for at least 1 month. Laser-induced remodeling was able to target MSC to damaged areas with ensuing specific increases in ocular progenitor cells. Thus, our results identify MSC and their secretum as crucial mediators of tissue repair in OAG through reactivation of local neural progenitors. In addition, laser treatment could represent an appealing strategy to promote MSC-mediated progenitor cell recruitment and tissue repair in chronic diseases.
