摘 要：RNA沉默是真核生物的一種高度保守的和序列特異的RNA降解系統(tǒng)，它不但是基礎(chǔ)生物學(xué)領(lǐng)域的研究熱點,，同時在調(diào)節(jié)基因表達或研究基因功能方面也是非常有前景的,。植物中的轉(zhuǎn)錄后基因沉默(PTGS)是RNA沉默的一種形式，通過PTGS能對目標RNA進行特異性降解,。對雙鏈RNA(dsRNA)在RNA沉默啟動中所起中心作用的認知,，形成了幾種RNA沉默載體的構(gòu)建方法，這些方法與基因組資源相結(jié)合,，通過轉(zhuǎn)基因或非轉(zhuǎn)基因的方法能夠快速和高效研究植物的基因功能,。
關(guān)鍵詞：RNA沉默；分析,；基因功能
The research on the analysis of gene function in plants mediated by RNA silencing
NIU Yan-Bing﹡, GUO Shi-Mi, SHEN Lin-Yan, LEI Wan-Jun, LEI Xiao-Fei
(Shanxi Agricultural University, Taigu 030801, China)
Abstract: Being a highly conserved and sequence-specific RNA degradation system in eukaryotic, RNA silencing is both a fundamental biological issue and a highly promising application upon the control of gene expression or the analysis of gene function. Posttranscriptional gene silencing (PTGS) in plants is a form of RNA silencing by which target RNA is degraded in a sequence-specific manner. Realizing regarding the central role that double-stranded RNA plays in triggering RNA silencing has prompted the development of several vectors construction for RNA silencing. These methods, in combination with the genomic resources, by means of transgenic and non-transgenic technology, have provided rapid and efficient means by which to investigate gene function in a wide range of plant species.
Key words: RNA silencing ; analysis; gene function
附件1下載: 20050830111429.pdf
