生物谷：研究人員用一種新技術(shù)來分析基因和蛋白質(zhì)的表達(dá)來獲得對衰老以及糖尿病的更詳細(xì)的信息,。以前，科學(xué)家曾了解帶有daf-2基因突變的美麗線蟲比沒有突變的線蟲的壽命長一倍,。daf-2降低一個名為“類胰島素信號發(fā)生”過程的活性,，從而延長了壽命。Meng-Qui Dong和同事用質(zhì)譜儀比較了帶有daf-2突變的線蟲和不帶突變的線蟲的蛋白質(zhì)的量,。他們識別出47個蛋白質(zhì)在突變線蟲中更豐富,，另有39個蛋白質(zhì)在突變線蟲中的量不豐富。實(shí)驗(yàn)顯示,，有些蛋白質(zhì)增加壽命,、有些降低壽命。Stuart K. Kim在一篇相關(guān)的研究評述中寫道：研究人員將繼續(xù)改進(jìn)這個方法,，因?yàn)檫@個技術(shù)現(xiàn)在只能用來研究線蟲基因組中不到10%的蛋白質(zhì),。
原始出處：
出處一：
Science 3 August 2007:
Vol. 317. no. 5838, pp. 660 - 663
DOI: 10.1126/science.1139952
Quantitative Mass Spectrometry Identifies Insulin Signaling Targets in C. elegans
Meng-Qiu Dong,1 John D. Venable,1 Nora Au,2,3 Tao Xu,1 Sung Kyu Park,1 Daniel Cociorva,1 Jeffrey R. Johnson,1 Andrew Dillin,2 John R. Yates, III1*
DAF-2, an insulin receptor–like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.
1 Scripps Research Institute, La Jolla, CA92037, USA.
2 Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
3 University of California at San Diego, La Jolla, CA 92093, USA.
* To whom correspondence should be addressed. E-mail: [email protected]
出處一：
Science 3 August 2007:
Vol. 317. no. 5838, pp. 603 - 604
DOI: 10.1126/science.1146805
CELL BIOLOGY:
Proteins That Promote Long Life
Stuart K. Kim
Quantitative analysis of proteins in a long-lived worm reveals changes that both increase and shorten life span
