生物谷:研究人員用一種新技術(shù)來(lái)分析基因和蛋白質(zhì)的表達(dá)來(lái)獲得對(duì)衰老以及糖尿病的更詳細(xì)的信息,。以前,,科學(xué)家曾了解帶有daf-2基因突變的美麗線(xiàn)蟲(chóng)比沒(méi)有突變的線(xiàn)蟲(chóng)的壽命長(zhǎng)一倍,。daf-2降低一個(gè)名為“類(lèi)胰島素信號(hào)發(fā)生”過(guò)程的活性,,從而延長(zhǎng)了壽命,。Meng-Qui Dong和同事用質(zhì)譜儀比較了帶有daf-2突變的線(xiàn)蟲(chóng)和不帶突變的線(xiàn)蟲(chóng)的蛋白質(zhì)的量,。他們識(shí)別出47個(gè)蛋白質(zhì)在突變線(xiàn)蟲(chóng)中更豐富,,另有39個(gè)蛋白質(zhì)在突變線(xiàn)蟲(chóng)中的量不豐富,。實(shí)驗(yàn)顯示,有些蛋白質(zhì)增加壽命,、有些降低壽命,。Stuart K. Kim在一篇相關(guān)的研究評(píng)述中寫(xiě)道:研究人員將繼續(xù)改進(jìn)這個(gè)方法,因?yàn)檫@個(gè)技術(shù)現(xiàn)在只能用來(lái)研究線(xiàn)蟲(chóng)基因組中不到10%的蛋白質(zhì),。
原始出處:
出處一:
Science 3 August 2007:
Vol. 317. no. 5838, pp. 660 - 663
DOI: 10.1126/science.1139952
Quantitative Mass Spectrometry Identifies Insulin Signaling Targets in C. elegans
Meng-Qiu Dong,1 John D. Venable,1 Nora Au,2,3 Tao Xu,1 Sung Kyu Park,1 Daniel Cociorva,1 Jeffrey R. Johnson,1 Andrew Dillin,2 John R. Yates, III1*
DAF-2, an insulin receptor–like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.
1 Scripps Research Institute, La Jolla, CA92037, USA.
2 Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
3 University of California at San Diego, La Jolla, CA 92093, USA.
* To whom correspondence should be addressed. E-mail: [email protected]
出處一:
Science 3 August 2007:
Vol. 317. no. 5838, pp. 603 - 604
DOI: 10.1126/science.1146805
CELL BIOLOGY:
Proteins That Promote Long Life
Stuart K. Kim
Quantitative analysis of proteins in a long-lived worm reveals changes that both increase and shorten life span