記者從煙臺獲悉,，由山東省干細胞工程技術研究中心,、煙臺毓璜頂醫(yī)院、煙臺大學李建遠教授率領的研究團隊,，經(jīng)過十年的潛心研究,，在人類精子成熟相關蛋白研究中取得重大突破，研究成果連續(xù)被美國國際著名學術期刊MCP《分子與細胞蛋白質組學》2010年11期和12月22日在線發(fā)表,。這標志著我國人類精子成熟相關研究進入了國際原創(chuàng)領先階段,。
據(jù)介紹，人類精子成熟是男性生殖調控的重要環(huán)節(jié),。精子在睪丸中產(chǎn)生,，通過附睪管腔微環(huán)境與附睪分泌蛋白相互作用發(fā)育成熟，為隨后的受精奠定基礎,。精子成熟障礙是引起男性不育的主要原因之一, 因此, 研究精子成熟相關蛋白有助于提高臨床不育的分子診斷及治療水平,。此外, 干擾精子成熟，還可以調控生殖有效避孕,。
李教授的研究團隊,，以源頭創(chuàng)新為起點，采用系統(tǒng)工程化分子生物學研究技術手段,，在成功解析了正常人附睪基因表達譜的基礎上,，又解析了人類附睪、睪丸蛋白表達譜,，附睪管腔液分泌型蛋白譜,，并建立了相應1030種蛋白抗體庫，鑒定發(fā)現(xiàn)附睪和睪丸表達的精子定位蛋白317種（包含了人附睪分泌精子結合蛋白家族）,，對其進行了科學系統(tǒng)的分類編號與命名,，并注冊于國際人類組織基因庫（GenBank）。這對于人們進一步了解精子上的定位蛋白的潛在功能提供了基礎,。該團隊通過功能研究發(fā)現(xiàn)了與精子運動,、穿卵、獲能,、抗氧化和免疫防御等功能有關的重要靶蛋白,。其所建立的人類精子成熟相關蛋白生物信息數(shù)據(jù)庫，通過網(wǎng)絡對國際開放，實現(xiàn)了信息資源共享,。
據(jù)悉,，山東省科技廳于2009年12月20日組織國內著名專家評審委員會對該成果進行了鑒定。評審委員會認為此成果具有原創(chuàng)性,、先進性,、科學性和實用性，達到國際領先水平,。（生物谷Bioon.com）
研究文章出處：
Molecular & Cellular Proteomics doi: 10.1074/mcp.M110.004630
Mapping of the human testicular proteome and its relationship with that of the epididymis and spermatozoa
JianYuan Li1,*, FuJun Liu1, Xin Liu1, Juan Liu1, Peng Zhu1, FengChun Wan2, ShaoHua Jin1, Ning Li1, Jie Liu1 and HaiYan Wang1
Abstract
The testis produces male gametes in the germinal epithelium through the development of spermatogonia and spermatocytes into spermatids and immature spermatozoa with the support of Sertoli cells. The flow of sperm into the epididymis is aided by testicular secretions. In the epididymal lumen, spermatozoa and testicular secretions combine with epididymal secretions that promote sperm maturation and storage. We refer to the combined secretions in the epididymis as the sperm-milieu. With 2D-PAGE MALDI-TOF/MS analysis of healthy testes from fertile accident victims, 725 unique proteins were identified from 1920 2D-gel spots, and a corresponding antibody library was established. This revealed the presence of 240 proteins in the sperm-milieu by Western blotting and the localisation of 167 proteins in mature spermatozoa by immuno-cytochemistry. These proteins, and those from the epididymal proteome (Li et al. 2010), form the proteomes of the sperm-milieu and the spermatozoa, comprising 525 and 319 proteins, respectively. Individual mapping of the 319 sperm-located proteins to various testicular cell types by immunohistochemistry suggested that 47% were intrinsic sperm proteins (from their presence in spermatids) and 23% were extrinsic sperm proteins, originating from the epididymis and acquired during maturation (from their absence from the germinal epithelium and presence in the epididymal tissue and sperm-milieu). Whereas 408 of 525 proteins in the sperm-milieu proteome were previously identified as abundant epididymal proteins, the remaining 22%, detected by the use of new testicular antibodies, were more likely to be minor proteins common to the testicular proteome, rather than proteins of testicular origin added to spermatozoa during maturation in the epididymis. The characterization of the sperm-milieu proteome and testicular mapping of the sperm-located proteins presented here provide the molecular basis for further studies on the production and maturation of spermatozoa. This could be the basis of development of diagnostic markers and therapeutic targets for infertility or male contraception.
