英國研究人員發(fā)現(xiàn)，連續(xù)缺覺一周擾亂700多個對健康至關(guān)重要的基因,，從而影響人體生物鐘,、新陳代謝,、免疫反應(yīng),、抗壓能力等,，意味著睡眠不佳可能對長期健康有廣泛影響,。
薩里大學(xué)薩里睡眠研究中心的德克-揚·迪克教授帶領(lǐng)研究小組,，征募14名男性和12名女性志愿者。志愿者年齡在23歲至31歲間,，身體健康,。研究人員要求志愿者在實驗室進(jìn)行兩個階段的睡眠實驗,，第一階段連續(xù)一周每晚躺在床上10個小時,，第二階段連續(xù)一周每晚躺床上6個小時,。每個階段結(jié)束后,，志愿者必須連續(xù)39至41個小時不睡覺。
研究人員在最新一期美國《國家科學(xué)院學(xué)報》（PNAS）發(fā)表論文說,，志愿者血樣檢測結(jié)果顯示,，睡眠時間對基因活動有大影響,，與睡眠充足時相比,，缺覺狀態(tài)下的志愿者，444個基因的活動遭抑制，267個基因更活躍,。
受到影響的包括控制新陳代謝的基因,，從而可能引起或加劇糖尿病、肥胖,；包括影響身體對炎癥反應(yīng)的基因,，可能影響心臟??；還包括一些關(guān)乎壓力和老化的基因,。這些變化有助了解缺覺者心臟病,、糖尿病、肥胖,、抑郁癥等疾病風(fēng)險增加的生物機制,。(生物谷Bioon.com)
doi: 10.1073/pnas.1217154110
PMC:
PMID:
Effects of insufficient sleep on circadian rhythmicity and expression amplitude of the human blood transcriptome
Carla S. Moller-Levet, Simon N. Archer, Giselda Bucca, Emma E. Laing, Ana Slak, Renata Kabiljo, June C. Y. Lo, Nayantara Santhi, Malcolm von Schantz, Colin P. Smith, and Derk-Jan Dijk.
Insufficient sleep and circadian rhythm disruption are associated with negative health outcomes, including obesity, cardiovascular disease, and cognitive impairment, but the mechanisms involved remain largely unexplored. Twenty-six participants were exposed to 1 wk of insufficient sleep (sleep-restriction condition 5.70 h, SEM = 0.03 sleep per 24 h) and 1 wk of sufficient sleep (control condition 8.50 h sleep, SEM = 0.11). Immediately following each condition, 10 whole-blood RNA samples were collected from each participant, while controlling for the effects of light, activity, and food, during a period of total sleep deprivation. Transcriptome analysis revealed that 711 genes were up- or down-regulated by insufficient sleep. Insufficient sleep also reduced the number of genes with a circadian expression profile from 1,855 to 1,481, reduced the circadian amplitude of these genes, and led to an increase in the number of genes that responded to subsequent total sleep deprivation from 122 to 856. Genes affected by insufficient sleep were associated with circadian rhythms (PER1, PER2, PER3, CRY2, CLOCK, NR1D1, NR1D2, RORA, DEC1, CSNK1E), sleep homeostasis (IL6, STAT3, KCNV2, CAMK2D), oxidative stress (PRDX2, PRDX5), and metabolism (SLC2A3, SLC2A5, GHRL, ABCA1). Biological processes affected included chromatin modification, gene-expression regulation, macromolecular metabolism, and inflammatory, immune and stress responses. Thus, insufficient sleep affects the human blood transcriptome, disrupts its circadian regulation, and intensifies the effects of acute total sleep deprivation. The identified biological processes may be involved with the negative effects of sleep loss on health, and highlight the interrelatedness of sleep homeostasis, circadian rhythmicity, and metabolism.
