決定RNA結(jié)合蛋白與其目標相互作用的RNA的序列和環(huán)境，過去往往是根據(jù)不同蛋白的情況逐一被研究的?，F(xiàn)在，由Timothy Hughes及其同事所完成的一項研究,，報告了對來自24種真核生物的超過200種RNA結(jié)合蛋白的結(jié)合點所做的一個全面分析。他們識別出了保守的RNA-結(jié)合主題,，同時他們的分析也允許僅僅根據(jù)“RNA-結(jié)合域”來預(yù)測相互作用點,。這些主題還被發(fā)現(xiàn)反映每個分子的功能,，這將有助于了解以前未被定性的先例所起作用,。（生物谷Bioon.com）
生物谷推薦英文摘要：
Nature doi:10.1038/nature12311
A compendium of RNA-binding motifs for decoding gene regulation
Debashish Ray, Hilal Kazan,Kate B. Cook, Matthew T. Weirauch,Hamed S. Najafabadi,Xiao Li,Serge Gueroussov,Mihai Albu,Hong Zheng, Ally Yang, Hong Na,Manuel Irimia,Leah H. Matzat,Ryan K. Dale,Sarah A. Smith,Christopher A. Yarosh, Seth M. Kelly,Behnam Nabet, Desirea Mecenas,Weimin Li,Rakesh S. Laishram, Mei Qiao, Howard D. Lipshitz, Fabio Piano,Anita H. Corbett et al.
RNA-binding proteins are key regulators of gene expression, yet only a small fraction have been functionally characterized. Here we report a systematic analysis of the RNA motifs recognized by RNA-binding proteins, encompassing 205 distinct genes from 24 diverse eukaryotes. The sequence specificities of RNA-binding proteins display deep evolutionary conservation, and the recognition preferences for a large fraction of metazoan RNA-binding proteins can thus be inferred from their RNA-binding domain sequence. The motifs that we identify in vitro correlate well with in vivo RNA-binding data. Moreover, we can associate them with distinct functional roles in diverse types of post-transcriptional regulation, enabling new insights into the functions of RNA-binding proteins both in normal physiology and in human disease. These data provide an unprecedented overview of RNA-binding proteins and their targets, and constitute an invaluable resource for determining post-transcriptional regulatory mechanisms in eukaryotes.
