美國克拉克森大學(xué)納米工程和生物技術(shù)實(shí)驗(yàn)室中心Igor Sokolov課題組開發(fā)出一種新的宮頸癌檢測(cè)方法——非特異性的硅珠黏附細(xì)胞法(nonspecific adhesion of silica beads to cells),。這篇研究報(bào)告發(fā)表在Small雜志上。
這項(xiàng)研究是基于課題組之前發(fā)表在Nature Nanotechnology雜志的研究報(bào)告,，他們觀察到,，正常的和發(fā)生癌變的宮頸上皮細(xì)胞表面存在著某些之前未發(fā)現(xiàn)的差異,。
在這項(xiàng)研究中，研究人員將硅珠連接到原子力顯微鏡(atomic force microscopy,AFM)的旋臂上,，從而使硅珠黏附在細(xì)胞表面,，硅珠和細(xì)胞分離所需的的黏附力的大小可以通過測(cè)量得到。黏附能力越高的細(xì)胞表面黏住的硅珠越多,。
根據(jù)細(xì)胞表面的熒光硅珠粒子的數(shù)目以及熒光的亮度輕易區(qū)別出癌細(xì)胞和正常細(xì)胞,。試驗(yàn)中所使用的超亮的熒光硅珠(ultrabright fluorescent silica particles)也是Sokolov課題組開發(fā)出來的。（生物谷Bioon.com）
生物谷推薦原始出處：
Small Volume 5 Issue 20, Pages 2277 - 2284
Towards Nonspecific Detection of Malignant Cervical Cells with Fluorescent Silica Beads
Swaminathan Iyer 1 2, Craig D. Woodworth 3 5, Ravi M. Gaikwad 1, Yaroslav Y. Kievsky 1 4, Igor Sokolov 1 5 *
1Department of Physics Clarkson University Potsdam, NY 13699 (USA)
2Department of Chemistry School of Biomedical, Biomolecular, and Chemical Sciences The University of Western Australia Crawley, WA (Australia)
3Department of Biology, NanoBio Laboratory (NABLAB) Clarkson University Potsdam, NY 13699 (USA)
4NRC Ottawa, ON, K1A 0R6 (Canada)
5Nanoengineering and Biotechnology Laboratories Center (NABLAB) Clarkson University Potsdam, NY 13699 (USA)vd
To date, the methods for detection of cancer cells are mostly based on traditional techniques used in biology, such as visual identification of malignant changes, cell-growth analysis, specific ligand-receptor labeling, or genetic tests. Despite being well developed, these methods are either insufficiently accurate or require a lengthy complicated analysis. A search for alternative methods for the detection of cancer cells may be a fruitful approach. Proposed here is a novel method for the detection of cancer cells in vitro, which is based on nonspecific adhesion of silica beads to cells. First, atomic force microscopy is used to study the adhesion of single silica beads to malignant and normal cells cultured from human cervix. It is found that adhesion depends on the time of contact, and can be statistically different for malignant and normal cells. Using these data, an optical method utilizing fluorescent silica beads is developed, which is based on detection of the difference in the number of adherent particles. The method is tested using primary cells cultured from cervical tissues of three healthy individuals and three patients with cervical cancer. The method shows sufficiently high sensitivity for cancer to make it interesting to perform further statistical tests.
