近日，一個國際研究小組通過研究揭示了一種新的標記物,，這種新的標記物對于我們理解癌癥在肝臟,、胰臟以及食道中的發(fā)展至關(guān)重要。相關(guān)研究成果刊登在了近日的國際雜志Stem Cell 上,。我們熟知的干細胞一般“居住”于器官中,，一般情況下，使干細胞分離非常困難,。研究者的這項研究揭示了一種抗體可以用于捕獲干細胞,。
研究者Pera教授表示，這種抗體可以檢測疾病狀態(tài)下的器官祖細胞,，比如肝硬化或者胰腺癌等癌癥,。通過識別這些祖細胞，研究者希望能夠?qū)W習到更多關(guān)于祖細胞在組織再生以及癌癥發(fā)展中所扮演的角色以及作用,。
研究者表示,，未來他們將開發(fā)出更多的抗體用于幫助檢測和識別以及分離組織中的干細胞，目前他們的研究對于諸如肝癌,、胰腺癌以及食道癌等的治療提供了很多思路,。（生物谷Bioon.com）
編譯自：Stem cell research aids understanding of cancer
doi:10.1002/stem.1167
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The GCTM-5 Epitope Associated with the Mucin-Like Glycoprotein FCGBP Marks Progenitor Cells in Tissues of Endodermal Origin†
Lincon A. Stamp1,2,‡, David R. Braxton3,‡, Jun Wu3,‡, Veronika Akopian3,‡, Kouichi Hasegawa3,‡, Parakrama T. Chandrasoma4, Susan M. Hawes1,2, Catriona McLean5, Lydia M. Petrovic4, Kasper Wang6, Martin F. Pera1,2,3,§,*
Monoclonal antibodies against cell surface markers are powerful tools in the study of tissue regeneration, repair and neoplasia, but there is a paucity of specific reagents to identify stem and progenitor cells in tissues of endodermal origin. The epitope defined by the GCTM-5 monoclonal antibody is a putative marker of hepatic progenitors. We sought to analyze further the distribution of the GCTM-5 antigen in normal tissues and disease states, and to characterize the antigen biochemically. The GCTM-5 epitope was specifically expressed on tissues derived from the definitive endoderm, in particular the fetal gut, liver and pancreas. Antibody reactivity was detected in subpopulations of normal adult biliary and pancreatic duct cells, and GCTM-5 positive cells isolated from the non-parenchymal fraction of adult liver expressed markers of progenitor cells. The GCTM-5 positive cell populations in liver and pancreas expanded greatly in numbers in disease states such as biliary atresia, cirrhosis and pancreatitis. Neoplasms arising in these tissues also expressed the GCTM-5 antigen, with pancreatic adenocarcinoma in particular showing strong and consistent reactivity. The GCTM-5 epitope was also strongly displayed on cells undergoing intestinal metaplasia in Barrett's esophagus, a precursor to esophageal carcinoma. Biochemical, mass spectrometry, and immunochemical studies revealed that the GCTM-5 epitope is associated with the mucin-like glycoprotein FCGBP. The GCTM-5 epitope on the mucin-like glycoprotein FCGBP is a cell surface marker for the study of normal differentiation lineages, regeneration, and disease progression in tissues of endodermal origin.
