葡萄糖轉(zhuǎn)運(yùn)蛋白是一類專門運(yùn)送葡萄糖的蛋白稱為葡萄糖轉(zhuǎn)運(yùn)蛋白。它一共分4種：葡萄糖轉(zhuǎn)運(yùn)蛋白1～4(Glut1～4),。Glut4是一種類胰島素敏感型葡萄糖運(yùn)輸載體,，它與2型糖尿病密切相關(guān)，細(xì)胞中Glut4表達(dá)的減少以及其轉(zhuǎn)位的障礙都是引發(fā)糖尿病的重要因素
早期研究發(fā)現(xiàn)葡萄糖轉(zhuǎn)運(yùn)蛋白-1（Glut-1）與惡性腫瘤的生物學(xué)行為密切相關(guān),。然而,，沒有任何證據(jù)闡明Glut -1的過度表達(dá)對(duì)癌細(xì)胞侵襲轉(zhuǎn)移機(jī)制。研究人員推測,，Glut -1調(diào)節(jié)膜型金屬蛋白酶-1（MT1-MMP）和基質(zhì)金屬蛋白酶-2（MMP-2）的表達(dá)促進(jìn)癌細(xì)胞侵襲運(yùn)動(dòng)。
采用RT-PCR分析Glut -1,、MMP-2,、β-肌動(dòng)蛋白,、MT1-MMP的表達(dá)。Western印跡法檢測Glut -1蛋白,、MMP-2,、MT1-MMP的表達(dá),。結(jié)果發(fā)現(xiàn)在Glut -1和MMP-2 mRNA和蛋白水平,，兩者共同表達(dá)于喉癌Hep-2喉癌細(xì)胞株中。
而在Hep-2喉癌細(xì)胞株轉(zhuǎn)染Glut -1反義寡核苷酸（AS-ODN）后,，MMP-2 mRNA和蛋白表達(dá)以及Glut -1 mRNA和蛋白表達(dá)出現(xiàn)下降。研究數(shù)據(jù)證實(shí)Glut -1和MMP-2的共表達(dá)在Hep-2喉癌細(xì)胞中,，Glut -1可調(diào)節(jié)MMP-2和MT1-MMP的表達(dá),。（生物谷：Bioon.com)
編譯自：Effect on the Expression of MMP-2, MT-MMP in Laryngeal Carcinoma Hep-2 Cell by Antisense Glucose Transporter-1
doi:10.1016/j.arcmed.2012.07.003
PMC:
PMID:
Effect on the Expression of MMP-2, MT-MMP in Laryngeal Carcinoma Hep-2 Cell by Antisense Glucose Transporter-1
Ying-Ying Xua, Yang-Yang Baoa, Shui-Hong Zhoua, Jun Fanb
Background and Aims
Glucose transporter protein-1 (Glut-1) is correlated with biological behaviors of malignant tumors. However, there was no evidence that overexpression of Glut-1 mechanistically lead to invasion or metastasis of cancer cells. We hypothesized that Glut-1 regulates the expression of membrane type 1-MMP (MT1-MMP) and matrix metalloproteinase-2 (MMP-2).
Methods
Analysis of the expression of Glut-1, MMP-2, β-actin, and MT1-MMP was performed using RT-PCR. Expression of Glut-1 protein, MMP-2, and MT1-MMP was detected by Western blotting.
Results
At mRNA and protein levels, Glut-1 and MMP-2 were co-expressed in the Hep-2 laryngeal carcinoma cell line. After transfection, Glut-1 antisense oligodeoxynucleotide (AS-ODN) decreased the expression of MMP-2 mRNA and protein as well as Glut-1 mRNA and protein. Glut-1 AS-ODN also decreased the expression of MT1-MMP mRNA.
Conclusions
Co-expression of Glut-1 and MMP-2 in Hep-2 laryngeal carcinoma cells and Glut-1 may regulate MMP-2 and MT1-MMP expression.
