近日,，刊登在國際雜志FASEB Journal上的一篇研究報告指出,，一種名為“flightless”的分子可以明顯幫助有效控制癌細胞在不同組織間轉(zhuǎn)移的速度。正是由于flightless分子的存在,，癌癥轉(zhuǎn)移的速度被有效的遏制了,。Flightless分子可以增加細胞之間的粘稠度，包括癌癥細胞,，這樣可以明顯減緩癌細胞擴散的速度,。
研究flightless以及其控制細胞運動的速度為未來控制癌癥的發(fā)展提供了新的思路,，研究者Christopher表示，我們希望有一天調(diào)節(jié)細胞運動的療法可以被用來控制癌細胞擴散速度,。
文章中,，研究者使用了三組細胞，一組細胞可以產(chǎn)生正常的flightless分子,，一組細胞不能產(chǎn)生，一組細胞可以高表達該分子,。隨后研究者研究了這些組細胞的運動比率,，并且檢測了這些細胞的特殊部分是否可以使細胞本身粘附于組織上面，結果顯示,，flightless分子在細胞運動遷移的速度上存在顯著性的效應,。
在一個器官中對付單一的腫瘤是非常困難的，但是許多癌癥的轉(zhuǎn)移為治療增加了新的障礙,，因此這項研究成果揭示了flightless分子對于阻止癌癥細胞遷移或者轉(zhuǎn)移速度,，進而抑制癌癥轉(zhuǎn)移，這無疑是癌癥病人治療的一大希望,。（生物谷Bioon.com）
編譯自：'Flightless' Molecule May Prevent Cancer from Spreading from One Tissue to Another
doi:10.1096/fj.11-202051
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Flightless I is a focal adhesion-associated actin-capping protein that regulates cell migration
Ibrahim Mohammad*, Pamma D. Arora*, Yeganeh Naghibzadeh*, Yongqiang Wang*, Jeff Li*, Wendall Mascarenhas*, Paul A. Janmey†, John F. Dawson‡ and Christopher A. McCulloch*,1
The role of adhesion-associated actin-binding proteins in cell migration is not well defined. In mouse fibroblasts we screened for focal adhesion-associated proteins that were isolated with collagen-coated beads and detected by tandem mass spectrometry. We identified flightless I (FliI) as an actin-binding protein in focal adhesion fractions, which was verified by immunoblotting. By confocal microscopy most FliI was distributed throughout the cytosol and in focal adhesions. By sedimentation assays and in vitro binding assays, we found that FliI associates with actin filaments and actin monomers. Assays using purified proteins showed that FliI inhibits actin polymerization and caps but does not sever actin filaments. Cells with FliI knockdown or cells overexpressing FliI migrated more or less rapidly, respectively, than wild-type controls. Compared with controls, cells with FliI knockdown were less adherent than wild-type cells, exhibited reduced numbers of focal adhesions containing activated β1 integrins and vinculin, and exhibited increased incorporation of actin monomers into nascent filaments at focal adhesions. These data indicate that FliI regulates cell migration through its localization to focal adhesions and its ability to cap actin filaments, which collectively affect focal adhesion maturation.—Mohammad, I., Arora, P. D., Naghibzadeh, Y., Wang, Y., Li, J., Mascarenhas, W., Janmey, P. A., Dawson, J. F., McCulloch, C. A. Flightless I is a focal adhesion-associated actin-capping protein that regulates cell migration.
