合成光感受器刺激和繼續(xù)暴露于光線照射,日本和卡爾斯魯厄研究將結(jié)果發(fā)表在Plant Cell期刊上,。
植物是依賴于太陽的,。陽光并不僅提供能量,同時(shí)也控制細(xì)胞的發(fā)育步驟,。所謂的光感受器激活發(fā)芽過程,、發(fā)展成葉片、花蕾形成等過程,。吸收光的感光組件可取代化學(xué)類似的合成物質(zhì)。
如果他們?cè)陂_發(fā)的植物在黑暗中Tilman Lamparter, Karlsruhe Institute of Technology (KIT) 研究人員說:阿拉伯芥的種子和幼苗送入名為“15EA-藻藍(lán)”的一種人工合成的物質(zhì),。這種物質(zhì)在植物細(xì)胞中,,可取代感光自然,光敏元件,。盡管在黑暗中,,模式植物的發(fā)芽和生長與暴露在光線下的對(duì)照組相似。
合成感光細(xì)胞可能是有價(jià)值的研究工具,,因?yàn)樗鼈兎奖闩c許多化工廠工藝即傳統(tǒng)的基因工程方法的研究相比較,。除了生長,光合作用也可以進(jìn)行研究好多,。 Lamparter預(yù)測(cè)花朵鮮花或光合作用系統(tǒng)的發(fā)展可能會(huì)在未來得到更好的控制,。 這些發(fā)現(xiàn)將使用高農(nóng)業(yè)產(chǎn)業(yè)化,在種植花卉或生物質(zhì)生產(chǎn)擁有實(shí)例,。(生物谷:Bioon.com)
doi:10.1105/tpc.111.094656
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PMID:
Assembly of Synthetic Locked Phycocyanobilin Derivatives with Phytochrome in Vitro and in Vivo in Ceratodon purpureus and Arabidopsis
Rui Yanga, Kaori Nishiyamab, Ayumi Kamiyab, Yutaka Ukajib, Katsuhiko Inomatab and Tilman Lampartera
Phytochromes are photoreceptors with a bilin chromophore in which light triggers the conversion between the red light–absorbing form, Pr, and the far-red-light–absorbing form, Pfr. Here we performed in vitro and in vivo studies using locked phycocyanobilin derivatives, termed 15 Z anti phycocyanobilin (15ZaPCB) and 15 E anti PCB (15EaPCB). Recombinant bacterial and plant phytochromes incorporated either chromophore in a noncovalent or covalent manner. All adducts were photoinactive. The absorption spectra of the 15ZaPCB and 15EaPCB adducts were comparable with those of the Pr and Pfr form, respectively. Feeding of 15EaPCB, but not 15ZaPCB, to protonemal filaments of the moss Ceratodon purpureus resulted in increased chlorophyll accumulation, modulation of gravitropism, and induction of side branches in darkness. The effect of locked chromophores on phytochrome responses, such as induction of seed germination, inhibition of hypocotyl elongation, induction of cotyledon opening, randomization of gravitropism, and gene regulation, were investigated in wild-type Arabidopsis thaliana and the phytochrome-chromophore–deficient long hypocotyl mutant hy1. All phytochrome responses were induced in darkness by 15EaPCB, not only in the mutant but also in the wild type. These studies show that the 15Ea stereochemistry of the chromophore results in the formation of active Pfr-like phytochrome in the cell. Locked chromophores might be used to investigate phytochrome responses in many other organisms without the need to isolate mutants. The induction of phytochrome responses in the hy1 mutant by 15EaPCB were however less efficient than by red light irradiation given to biliverdin-rescued seeds or seedlings.