Programmable Cells: Engineer Turns Bacteria Into Living Computers

04/27/05 -- In a step toward making living cells function as if they were tiny computers, engineers at Princeton have programmed bacteria to communicate with each other and produce color-coded patterns.

The feat, accomplished in a biology lab within the Department of Electrical Engineering, represents an important proof-of-principle in an emerging field known as "synthetic biology," which aims to harness living cells as workhorses that detect hazards, build structures or repair tissues and organs within the body.

"We are really moving beyond the ability to program individual cells to programming a large collection -- millions or billions -- of cells to do interesting things," said Ron Weiss, an assistant professor of electrical engineering and molecular biology.

Collaborating with researchers at the California Institute of Technology, Weiss and graduate student Subhayu Basu programmed E. coli bacteria to emit red or green fluorescent light in response to a signal emitted from another set of E. coli. In one experiment, the cells glowed green when they sensed a higher concentration of the signal chemical and red when they sensed a lower concentration. In a Petri dish, they formed a bull's-eye pattern -- a green circle inside a red one -- surrounding the sender cells.

In addition to demonstrating that the genetic programming techniques work, this sensing system could be useful for the detection of chemicals or organisms in laboratory tests. "The bull's-eye could tell you: This is where the anthrax is," said Weiss.

The researchers published their results in the April 28 issue of Nature. In addition to Weiss and Basu, authors of the paper are postdoctoral researcher Yoram Gerchman at Princeton and professor of chemical engineering Frances Arnold and graduate student Cynthia Collins at Caltech. It was funded by a grant from the U.S. Defense Advanced Research Projects Agency.

In previous work, including a paper published March 8 in the Proceedings of the National Academy of Sciences along with Sara Hooshangi and Stephan Thiberge, Weiss showed the feasibility of inserting engineered pieces of DNA into cells to make them behave in the same manner as digital circuits. The cells, for example, could be made to perform basic mathematical logic and produce crisp, reliable readouts that are more commonly associated with silicon chips than biological organisms. The new paper applies similar techniques to a large population of cells.

"Here we're showing an integrated package where the cells have an ability to send messages and other cells have the ability to act on these messages," said Weiss.

The creation of patterns, such as the bull's-eye effect, is a key step in one of Weiss' eventual goals, which is to have the cells secrete materials that build physical devices such as antennas or transmitters in places that are hard for humans to reach. Programmed cells also could be used to control the repair or construction of tissues within the body, possibly guiding stem cells to the locations where they are needed for the growth of new nerve or bone cells in a process Weiss called "programmed tissue engineering."

Even the early step of creating patterns in a Petri dish, however, may be useful as a tool for other scientists, particularly developmental biologists who are trying to understand how the cells of an embryo arrange themselves into patterns that become the various body parts of a mature organism. In fruit fly embryos, for example, the first cells are thought to differentiate into the head, abdomen and other parts based on the concentration of chemical signals that are emitted from the ends of the embryo.

In addition to conducting laboratory experiments, Weiss and colleagues are creating computer models of their engineered systems, which allow them to study how small modifications would affect the ultimate behavior of the organisms. So far, said Weiss, the experimental results have matched the computer models fairly closely, but the goal is to have a mathematically exact description of how each component works.

"One of the nice things about synthetic biology is that because we built the network from scratch, we should be able to model all the important details," he said. At some point in the future, he said, scientists will be able to choose a behavior they want from cells, and a computer program will create a genetic circuit to accomplish the task. "Then we can do an experiment to see if the community of cells is behaving as we desire. That is going to have a tremendous number of applications."

Source: Princeton University

　　據(jù)生物網(wǎng)4月27日消息,，美國(guó)普林斯頓大學(xué)的研究人員使細(xì)胞相互“交流”并產(chǎn)生彩色編碼樣式,。這一研究代表了“合成生物學(xué)”這一新興領(lǐng)域處于原理論證階段,。合成生物學(xué)旨在利用活體細(xì)胞來(lái)探測(cè)人體內(nèi)的危險(xiǎn)物質(zhì),、修補(bǔ)受損組織和器官,。

　　電子工程學(xué)和分子生物學(xué)助理教授Ron Weiss與加州理工學(xué)院的研究人員合作,，使E. coli細(xì)菌在另一組E. coli細(xì)菌發(fā)出信號(hào)的刺激下產(chǎn)生紅或綠的熒光,。當(dāng)E. coli細(xì)菌感覺到較高濃度的化學(xué)信號(hào)時(shí),，細(xì)胞發(fā)出綠光,；若濃度較低時(shí),，則發(fā)出紅光。在皮氏培養(yǎng)皿中,，研究人員發(fā)現(xiàn)細(xì)胞呈靶形（中間一個(gè)紅的,，外面一圈綠的）。除了可以證明基因編碼的工作機(jī)制外,，這一感應(yīng)體系還能用于實(shí)驗(yàn)室檢測(cè)化學(xué)物質(zhì)或有機(jī)體,。

　　這一研究得到美國(guó)國(guó)防部高級(jí)研究計(jì)劃署的資助，研究成果發(fā)表于2005年4月28日的《自然》雜志上,。

　　先前的研究中,，Weiss說(shuō)明了將DNA插入細(xì)胞使其以同一方式運(yùn)作的可行性。比如,，細(xì)胞可以按照基本的數(shù)理邏輯運(yùn)作,，這與生物體相比更接近于硅片的模式。現(xiàn)在,，研究人員將相同的技術(shù)運(yùn)用于大量的細(xì)胞中,。Weiss說(shuō)：“我們?cè)谧龅木褪且@示細(xì)胞發(fā)出信息的能力，和其他細(xì)胞對(duì)信息做出回應(yīng)的能力,。”

　　靶心效應(yīng)的產(chǎn)生對(duì)研究人員來(lái)說(shuō)是達(dá)到最終目標(biāo)的關(guān)鍵一步,。細(xì)胞還可被用來(lái)控制人體內(nèi)受損組織的修復(fù)，將干細(xì)胞引導(dǎo)到需要生長(zhǎng)新的神經(jīng)細(xì)胞的地方,。

　　皮氏培養(yǎng)皿中的發(fā)現(xiàn)對(duì)其他科學(xué)家可能有用,，特別是那些試圖了解胚胎細(xì)胞如何成為成熟生物體不同身體部分的生物學(xué)家們。

　　Weiss及其同事們正將他們的研究成果制成計(jì)算機(jī)模型,，這樣方便他們研究小的修改是如何影響生物體的最終表現(xiàn),。到目前為止,，實(shí)驗(yàn)結(jié)果與計(jì)算機(jī)模型非常吻合，但最終目標(biāo)是精確描述各個(gè)組成的運(yùn)作模式,。

　　將來(lái)也許科學(xué)家可以隨意選擇一種他們想要的細(xì)胞行為,，然后計(jì)算機(jī)程序?qū)⒅瞥苫螂娐穲D來(lái)幫助完成任務(wù)。