最近，科學(xué)家發(fā)現(xiàn)脂肪細(xì)胞中的蛋白會(huì)在志愿者體重下降的情況下發(fā)生了變化,。這些蛋白可能會(huì)成為用于監(jiān)控或改善低熱量飲食效果的指標(biāo),。研究成果刊登在美國(guó)化學(xué)學(xué)會(huì)(ACS)旗下《蛋白質(zhì)組研究雜志》(Journalof Proteome Research)網(wǎng)站上。
領(lǐng)導(dǎo)實(shí)施這項(xiàng)研究的愛(ài)德溫-馬里曼(Edwin Mariman)及其同事指出,，科學(xué)家很久以前便清楚,，嚴(yán)格限制熱量攝入的同時(shí)維持良好的營(yíng)養(yǎng)補(bǔ)劑，可以使動(dòng)物活得更長(zhǎng)久,，而且更健康,。之前的研究表明，人類(lèi)這樣做可能會(huì)獲得類(lèi)似的效果,。但是,，科學(xué)家對(duì)這些飲食習(xí)慣對(duì)人類(lèi)的作用機(jī)制尤其是對(duì)存儲(chǔ)脂肪的細(xì)胞的作用知之甚少。
最新研究聚焦于超重志愿者的腹部皮下脂肪細(xì)胞中的蛋白質(zhì),，他們堅(jiān)持了為期5周的低熱量飲食習(xí)慣,，每人平均減掉了21磅(約合9公斤)的體重?？茖W(xué)家發(fā)現(xiàn)有6種蛋白會(huì)隨志愿者體重減輕而發(fā)生變化,，其中包括調(diào)節(jié)人體存儲(chǔ)脂肪的蛋白。研究人員表示,，這些蛋白可以作為用于改善或追蹤低熱量飲食效果的重要指標(biāo),。（生物谷Bioon.com）
生物谷推薦原始出處：
J. Proteome Res., 2009, 8 (12), pp 5532–5540 DOI: 10.1021/pr900606m
The Physiologic Effects of Caloric Restriction Are Reflected in the in Vivo Adipocyte-Enriched Proteome of Overweight/Obese Subjects
Freek G. Bouwman, Mandy Claessens, Marleen A. van Baak, Jean-Paul Noben, Ping Wang, Wim H. M. Saris and Edwin C. M. Mariman*
NUTRIM School for Nutrition, Toxicology and Metabolism, Department of Human Biology, Maastricht University Medical Centre+, P.O. Box 616, NL-6200MD Maastricht, The Netherlands, and Hasselt University, Biomedical Research Institute and Transnational University Limburg, School of Life Sciences, Diepenbeek, Belgium
We have applied our recently designed proteomics apparoach to search for protein changes in the in vivo adipocyte-enriched proteome from 8 overweight/obese subjects who underwent an intervention of 5 weeks of a very low calorie diet followed by 3 weeks of a normal diet. On average, persons lost 9.5 kg body weight largely contributed by the loss of fat mass (7.1 kg). Various parameters of adiposity and lipid metabolism changed significantly. Proteomics analysis revealed 6 significantly changed proteins. Analysis indicates that fructose-bisphosphate aldolase C and tubulin beta 5 are potential biomarkers for the present intervention. Further, identified proteins indicate a reduced intracellular scaffolding of GLUT4 (ALDOC, TUBB5, ANXA2), an increased uptake of fatty acids (FABP4), an improved inflammatory profile of the adipose tissue (ApoA1, AOP1) and a change in fat droplet organization (vimentin). Correlation analysis between changes in protein spot intensities and parameters of adiposity or lipid metabolism points to a link between aldo-ketoreductase 1C2 and parameters of adiposity, between FABP4 and parameters of lipid metabolism, and between proteins for beta-oxidation (HADH, ACADS, ACAT1) and FFA levels. Altogether, our findings underscore the potential value of in vivo proteomics for human intervention studies.
