近日,，中科院植物研究所沈世華研究組通過蛋白質(zhì)組學(xué)、電鏡技術(shù)等全面解析了世界著名生物柴油樹種的油脂動(dòng)員網(wǎng)絡(luò),，描繪出胚乳中貯存油脂動(dòng)員的亞細(xì)胞超微結(jié)構(gòu)及其相關(guān)代謝酶類構(gòu)成的過程圖譜,。該成果發(fā)表在《蛋白質(zhì)組學(xué)研究雜志》（Journal of Proteome Research）（2009, 8: 1441-1451）雜志上。
麻瘋樹 (Jatropha curcas L.),，又名小桐子,，多年生灌木或小喬木，種子富含油脂，是生產(chǎn)生物柴油的理想原料,，近年來其開發(fā)利用得到全世界的極大重視,。麻瘋樹油脂主要儲(chǔ)存于成熟種子的肥厚胚乳中。通過蛋白質(zhì)雙向電泳,、質(zhì)譜技術(shù)、電鏡技術(shù)和生化等手段,，分析種子萌發(fā)過程中胚乳脂類及其脂肪酸構(gòu)成,、亞細(xì)胞超微結(jié)構(gòu)、蛋白質(zhì)組等的變化和關(guān)系,，發(fā)現(xiàn)隨著油體的解體和油脂的動(dòng)員,，至少50個(gè)胚乳蛋白質(zhì)呈現(xiàn)動(dòng)態(tài)變化，其中17個(gè)蛋白點(diǎn)直接參與了油脂動(dòng)員過程,，包括β氧化,、乙醛酸循環(huán)、糖酵解,、檸檬酸循環(huán),、糖異生、戊糖磷酸途徑等構(gòu)成了脂肪酸動(dòng)員和利用的代謝網(wǎng)絡(luò),。同時(shí),，根據(jù)脂肪酸組成的變化特點(diǎn)，推斷至少有2個(gè)途徑參與了油體中三酯酰甘油的動(dòng)員過程,。
該研究成果對(duì)油脂代謝基礎(chǔ)理論,、麻瘋樹新品種培育以及生物柴油的開發(fā)利用都有重要意義。（生物谷Bioon.com）
生物谷推薦原始出處：
J. Proteome Res., 2009, 8 (3), DOI: 10.1021/pr800799s
Proteomic Analysis of Oil Mobilization in Seed Germination and Postgermination Development of Jatropha curcas
Ming-Feng Yang#, Yu-Jun Liu#, Yun Liu, Hui Chen*, Fan Chen and Shi-Hua Shen*
Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, P. R. China and Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, Beijing 100080, P. R. China
To understand oil mobilization in germinating seeds, we performed ultrastructural observation and proteomic analysis of endosperm in germinating Jatropha curcas seeds. Results showed that the oil mobilization was initiated during germination, and then the oil was consumed for early seedling development. The significant change in abundance of 50 protein spots during germination indicated that several pathways including β-oxidation, glyoxylate cycle, glycolysis, citric acid cycle, gluconeogenesis, and pentose phosphate pathway were involved in the oil mobilization.
