生物谷報(bào)道：近日,，太平洋西北部國家實(shí)驗(yàn)室的科學(xué)家通過蛋白質(zhì)組學(xué)的方法,，已經(jīng)在瘟疫細(xì)菌--鼠疫耶氏菌上發(fā)現(xiàn)了176種蛋白以及可能的蛋白,。鼠疫耶氏菌的數(shù)量是隨著疾病的致病力而上升,、下降的,。
由能源實(shí)驗(yàn)室的科學(xué)家Mary Lipton 和 Kim Hixson帶領(lǐng)的團(tuán)隊(duì),，根據(jù)蛋白組學(xué)研究雜志最新問題的研究，認(rèn)為這些蛋白質(zhì)是與生長條件明確相關(guān)的獨(dú)特的生物標(biāo)記,。這種與疾病進(jìn)展聯(lián)系的生物標(biāo)記有望作為公共衛(wèi)生和生物防御的探測工具,，也可以指導(dǎo)藥物和疫苗設(shè)計(jì)者在他們的探索中破壞微生物的感染能力。
鼠疫耶氏菌是導(dǎo)致可怕的黑死病瘟疫的細(xì)菌,。跳蚤是這種病的細(xì)菌攜帶者并且可以將其傳播至嚙鼠動(dòng)物和人類宿主。這個(gè)研究模擬了鼠疫耶氏菌在跳蚤和哺乳動(dòng)物內(nèi)的環(huán)境條件,。
蛋白質(zhì)組是對(duì)一個(gè)細(xì)胞內(nèi)所有蛋白質(zhì)的縱覽,。Lipton, Hixson和他們的同事在PNNL-based Environmental Molecular Sciences Laboratory and Lawrence Livermore National Laboratory這兩個(gè)實(shí)驗(yàn)用精確質(zhì)量的蛋白組學(xué)技術(shù)、帶時(shí)間標(biāo)記的質(zhì)譜分析法和聚集性分析法來比較在四種不同的生長條件下的992種蛋白的豐富的變化,。這四種生長條件分別是：26攝氏度,，37攝氏度，加鈣和不加鈣,。
他們發(fā)現(xiàn)了與已知29種毒力相關(guān)蛋白具有相似多變化的89種候選蛋白,，還有另外87種與疾病條件關(guān)聯(lián)的“假設(shè)”蛋白?；蚪M研究機(jī)構(gòu)將假設(shè)蛋白定義為一種有基因檢測規(guī)則鑒定但不與任何已知蛋白序列相匹配或沒有其它證據(jù)證明它就是一個(gè)基因產(chǎn)物的蛋白,。
這項(xiàng)研究的作者說，在更加廣泛的生物系統(tǒng),，從另外一種傳染源如沙門氏菌到對(duì)凈化有毒廢棄物有興趣的廢物微生物,，也可以應(yīng)用同樣的方法用來尋找生物標(biāo)記。
原文出處：
Plague Proteome Reveals Proteins Linked to Infection
11/22/06 -- Recreating growth conditions in flea carriers and mammal hosts, Pacific Northwest National Laboratory scientists have uncovered 176 proteins and likely proteins in the plague-bacterium Yersinia pestis whose numbers rise and fall according to the disease?s virulence.
The team, led by the Department of Energy laboratory staff scientists Mary Lipton and Kim Hixson, identified the proteins as "unique biomarkers related specifically to growth condition," according to a study in the latest issue of the Journal of Proteome Research. Biomarkers associated with disease progression show promise as detection tools in public health and biodefense and can guide drug and vaccine designers in their quest to disrupt the microbe?s ability to infect.
Y. pestis is the bacterium that caused the infamous Black Death plagues. Fleas are vectors for the disease and can spread it to rodent and human hosts. This study mimicked environmental conditions of Y. pestis in flea and in mammalian systems.
The proteome is a survey of proteins in a cell. Lipton, Hixson and colleagues at the PNNL-based Environmental Molecular Sciences Laboratory and Lawrence Livermore National Laboratory used proteomic techniques called accurate mass and time tag mass spectrometry and clustering analysis to compare abundance changes in 992 proteins under four different growth conditions, at 26 degrees and 37 degrees Celsius and with and without calcium.
They found 89 candidate proteins with similar abundance changes to 29 known virulence-linked proteins, and an additional 87 disease-condition-associated "hypothetical" proteins. The Institute for Genomic Research defines a hypothetical protein as one identified by a gene-finding algorithm that matches no other known protein sequence or contains no other evidence that it is an actual product of a gene.
The study authors said the same approach is being applied to a search for biomarkers across a wide range of biological systems, from other infectious agents such as Salmonella to soil microbes of interest in cleaning up toxic waste.
Source: DOE/Pacific Northwest National Laboratory
http://www.bio.com/newsfeatures/newsfeatures_research.jhtml?cid=23900042
