生物谷報(bào)道：隨著蛋白質(zhì)組學(xué)的發(fā)展,，大規(guī)模研究蛋白間相互作用已成為重要的研究方向之一,。這篇是剛剛出版的PNAS上一篇報(bào)道,，作用研究了酵母膜表面705種蛋白,，以及其中536種蛋白間的至少1985種相互聯(lián)系和作用,。這種研究為今后更廣泛的物種的蛋白質(zhì)間相互作用提供示范,。

原文鏈接：

http://www.pnas.org/cgi/content/full/102/34/12123

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We carried out a large-scale screen to identify interactions between integral membrane proteins of Saccharomyces cerevisiae by using a modified split-ubiquitin technique. Among 705 proteins annotated as integral membrane, we identified 1,985 putative interactions involving 536 proteins. To ascribe confidence levels to the interactions, we used a support vector machine algorithm to classify interactions based on the assay results and protein data derived from the literature. Previously identified and computationally supported interactions were used to train the support vector machine, which identified 131 interactions of highest confidence, 209 of the next highest confidence, 468 of the next highest, and the remaining 1,085 of low confidence. This study provides numerous putative interactions among a class of proteins that have been difficult to analyze on a high-throughput basis by other approaches. The results identify potential previously undescribed components of established biological processes and roles for integral membrane proteins of ascribed functions.