生物谷報(bào)道：在過(guò)去的十年里,，芯片技術(shù)已經(jīng)在基因表達(dá)研究中起到了舉足輕重的作用,，帶來(lái)了深廣的影響?；蛐酒玫胶Ａ繑?shù)據(jù),，但這些海量數(shù)據(jù)是否準(zhǔn)確？如何最大限度提高芯片數(shù)據(jù)的準(zhǔn)確性,，是科學(xué)家們最頭疼的問(wèn)題,。尤其是但是隨著利用不同芯片平臺(tái)獲得相異甚至大相徑庭的結(jié)果逐漸增多,，許多人產(chǎn)生了對(duì)這一技術(shù)可靠性的質(zhì)疑爭(zhēng)論。芯片質(zhì)量控制（MicroArray Quality Control,，MAQC）計(jì)劃因而得以誕生,，作為一個(gè)標(biāo)準(zhǔn)將對(duì)相關(guān)數(shù)據(jù)分析和爭(zhēng)論進(jìn)行評(píng)定。
最新著名的基因芯片專(zhuān)家,，華人學(xué)者石樂(lè)明（音譯）以同一期Nature biotech 上發(fā)表了5篇文章系統(tǒng)闡述有關(guān)基因芯片質(zhì)量控制的問(wèn)題解決。
外源RNA對(duì)照（External RNA controls,，ERCs)是MAQC的一個(gè)重要部分,，對(duì)于芯片分析性能測(cè)評(píng)也是非常重要的一個(gè)指標(biāo)，但是在各研究團(tuán)體中并未能得到充分利用,。在這一研究報(bào)告中,，研究人員對(duì)兩類(lèi)ERCs進(jìn)行了評(píng)估：其中一類(lèi)在樣品擴(kuò)增和標(biāo)記之間加入到總RNA中，另外一類(lèi)怎是在雜交之前加入到copyRNAs (cRNAs)中,，通過(guò)多商業(yè)芯片平臺(tái)（multiple commercial microarray platforms獲得了ERC濃度反應(yīng)曲線(xiàn)（concentration-response curves）,，辨認(rèn)出在分析過(guò)程中出現(xiàn)問(wèn)題的分析以及潛在的變量來(lái)源，除此之外研究人員也對(duì)不同的ERC類(lèi)型的行為進(jìn)行了檢測(cè),。這些ERC評(píng)估將為未來(lái)的多平臺(tái)分析性能評(píng)估中ERC的更好使用提供了一個(gè)基礎(chǔ),。在這一研究中，浙江大學(xué)部分研究工作者參與了這一計(jì)劃,。
Dr. Leming Shi
CURRENT EMPLOYMENT
Computational Chemist, NCTR/FDA, April 2003 – Present
Responsibilities: Microarray quality control, chemoinformatics, bioinformatics, and systems toxicology
EDUCATION
· Ph.D. in Computational Chemistry (1991), Chinese Academy of Sciences, Beijing, China.
· M.Sc. in Computational Chemistry and Chemometrics (1988), University of Science and Technology of China, Hefei, China.
· B.Sc. in Analytical Chemistry (1985), Hunan University, Changsha, China.
EMPLOYMENT HISTORY
· Chipscreen Biosciences, Ltd., Shenzhen, China. Co-founder and Director of Informatics (5/2001-4/2003).
· BASF Corp, Princeton, New Jersey. Senior Scientist (7/2000-5/2001).
· American Home Products Corp. (now Wyeth), Princeton, New Jersey. Senior Research Chemist (8/1999-6/2000).
· R.O.W. Sciences, Inc. at the NCTR/FDA, Jefferson, Arkansas. Computational Chemist (12/1997-8/1999).
· National Cancer Institute, NIH, Bethesda, Maryland. Visiting Fellow (9/1995-12/1997).
· Case Western Reserve University, Department of Chemistry, Cleveland, Ohio. Research Associate (8/1994-9/1995).
· Chinese Academy of Sciences, Institute of Process Engineering, Beijing, China. Assistant and Associate Research Professor (7/1991-8/1994).
Dr. Shi co-authored about 100 peer-reviewed publications in chemoinformatics, bioinformatics, and data mining. His current research focus is coordinating the MAQC (MicroArray Quality Control) project, a large, community-wide effort aimed at standardizing the microarray technology in terms of quality control and data analysis(http://www.fda.gov/nctr/science/centers/toxicoinformatics/maqc/). Results from the first phase of the MAQC project have been recently published in a series of papers in the September, 2006 issue of Nature Biotechnology.
(http://www.nature.com/nbt/focus/maqc/index.html).
