中科院北京基因組研究所的胡松年研究員和浙江大學(xué)醫(yī)學(xué)院附屬二院的胡訊教授合作研究的項(xiàng)目“細(xì)菌Phenylobacterium zucineum全基因組測(cè)序及分析”取得階段性成果,，相關(guān)論文發(fā)表在今年第9期的BMC Genomics上。
P.zucineum是合作方從K562細(xì)胞系中分離到的兼性細(xì)菌,。系統(tǒng)分類學(xué)上，該細(xì)菌屬于Phenylobacterium,。早期研究者發(fā)現(xiàn)Phenylobacterium屬細(xì)菌可以把苯丙氨酸(phenylalanine)作為唯一碳源，故將該細(xì)菌屬命名為Phenylobacterium,。P.zucineum基因組含有苯丙氨酸代謝通路的所有關(guān)鍵基因，這為Phenylobacterium屬細(xì)菌利用苯丙氨酸提供了遺傳依據(jù),。P.zucineum能在細(xì)胞外自由生長(zhǎng),，又能在體外培養(yǎng)的細(xì)胞系中長(zhǎng)期生存,，具有強(qiáng)大的環(huán)境適應(yīng)能力。P.zucineum基因組編碼大量的細(xì)胞外周sigma作用因子,，轉(zhuǎn)錄調(diào)節(jié)蛋白,，雙相信號(hào)傳遞蛋白和熱激分子伴侶等環(huán)境適應(yīng)相關(guān)蛋白,。這些蛋白的鑒定很好地解釋了P.zucineum所擁有的強(qiáng)大環(huán)境適應(yīng)能力。
另外,，作為Phenylobacterium屬中唯一具有全基因組序列的細(xì)菌,，P.zucineum基因組序列將為細(xì)菌比較基因組學(xué)研究提供有價(jià)值的數(shù)據(jù)基礎(chǔ),。 （生物谷Bioon.com）
生物谷推薦原始出處：
BMC Genomics 2008, 9:386doi:10.1186/1471-2164-9-386
Complete genome of Phenylobacterium zucineum – a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562
Yingfeng Luo 1,2,3 , Xiaoli Xu* 1 , Zonghui Ding* 1 , Zhen Liu1 , Bing Zhang2,3 , Zhiyu Yan1 , Jie Sun1 , Songnian Hu2,3 and Xun Hu1
1 Cancer Institute (Key Laboratory for Cancer Intervention and Prevention, National Ministry of Education, PR China; Key Laboratory of Molecular Biology in Medical Sciences, Zhejiang Province, PR China), the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, PR China
2 James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou, PR China
3 Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, PR China
ackground
Phenylobacterium zucineum is a recently identified facultative intracellular species isolated from the human leukemia cell line K562. Unlike the known intracellular pathogens, P. zucineum maintains a stable association with its host cell without affecting the growth and morphology of the latter.
Results
Here, we report the whole genome sequence of the type strain HLK1T. The genome consists of a circular chromosome (3,996,255 bp) and a circular plasmid (382,976 bp). It encodes 3,861 putative proteins, 42 tRNAs, and a 16S-23S-5S rRNA operon. Comparative genomic analysis revealed that it is phylogenetically closest to Caulobacter crescentus, a model species for cell cycle research. Notably, P. zucineumhas a gene that is strikingly similar, both structurally and functionally, to the cell cycle master regulator CtrA of C. crescentus, and most of the genes directly regulated by CtrA in the latter have orthologs in the former.
Conclusion
This work presents the first complete bacterial genome in the genus Phenylobacterium. Comparative genomic analysis indicated that the CtrA regulon is well conserved between C. crescentus and P. zucineum.
