來自中國農(nóng)業(yè)大學(xué)，國家植物基因研究中心,，植物生理與生物化學(xué)國家重點(diǎn)實(shí)驗(yàn)室的研究人員利用Affymetrix擬南芥ATH1基因組芯片,，全面分析了花粉萌發(fā)和花粉管生長過程中的轉(zhuǎn)錄組變化情況,，發(fā)現(xiàn)了這個(gè)過程中一些重要變化,。這一研究成果公布在Plant Physiol.雜志上。
花粉萌發(fā)（Pollen germination）即花粉沿著花粉管的生長過程,，是開花植物繁殖的一個(gè)必要過程,，這個(gè)過程是研究細(xì)胞生長和形態(tài)發(fā)生的一個(gè)理想模型。
為了能進(jìn)一步了解這個(gè)重要過程的詳細(xì)細(xì)節(jié),，研究人員在這篇文章中全面分析了花粉萌發(fā)和花粉管生長過程中的轉(zhuǎn)錄組變化情況,。通過利用Affymetrix擬南芥ATH1基因組芯片，研究人員首次發(fā)現(xiàn)了從脫水成熟花粉粒,，到含水花粉粒,，再到擬南芥的花粉管的轉(zhuǎn)錄組變化：這些花粉和花粉管中表達(dá)基因的數(shù)目，無論是整個(gè)表達(dá)基因,，還是特異性表達(dá)的基因,，都增加了很多。
Gene Ontology的分析結(jié)果表明,，在花粉萌發(fā)和花粉管生長過程中,，與細(xì)胞存活，轉(zhuǎn)錄,，信號(hào)傳導(dǎo)和細(xì)胞轉(zhuǎn)運(yùn)有關(guān)的表達(dá)基因發(fā)生了很大的變化,，尤其是那些正調(diào)控的基因,。而且研究人員還發(fā)現(xiàn)，類鈣調(diào)蛋白,，陽離子交換因子，以及熱激蛋白家族在花粉萌發(fā)和花粉管生長過程中變化較大,，這些結(jié)果都說明在這個(gè)過程中,，基因的整體轉(zhuǎn)錄都增加了。
在花粉萌發(fā)和花粉管過程中新出現(xiàn)的這些轉(zhuǎn)錄組說明這個(gè)復(fù)雜過程中出現(xiàn)了新的基因表達(dá),，這些新表達(dá)的基因的功能值得更加深入的研究,。（生物谷Bioon.com）
生物谷推薦原始出處：
Plant Physiology 148:1201-1211 (2008) DOI:10.1104/pp.108.126375
Transcriptome Analyses Show Changes in Gene Expression to Accompany Pollen Germination and Tube Growth in Arabidopsis
Yi Wang2, Wen-Zheng Zhang2, Lian-Fen Song, Jun-Jie Zou, Zhen Su and Wei-Hua Wu*
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, National Plant Gene Research Center (Beijing), China Agricultural University, Beijing 100193, China
Pollen germination, along with pollen tube growth, is an essential process for the reproduction of flowering plants. The germinating pollen with tip-growth characteristics provides an ideal model system for the study of cell growth and morphogenesis. As an essential step toward a detailed understanding of this important process, the objective of this study was to comprehensively analyze the transcriptome changes during pollen germination and pollen tube growth. Using Affymetrix Arabidopsis (Arabidopsis thaliana) ATH1 Genome Arrays, this study is, to our knowledge, the first to show the changes in the transcriptome from desiccated mature pollen grains to hydrated pollen grains and then to pollen tubes of Arabidopsis. The number of expressed genes, either for total expressed genes or for specifically expressed genes, increased significantly from desiccated mature pollen to hydrated pollen and again to growing pollen tubes, which is consistent with the finding that pollen germination and tube growth were significantly inhibited in vitro by a transcriptional inhibitor. The results of Gene Ontology analyses showed that expression of genes related to cell rescue, transcription, signal transduction, and cellular transport was significantly changed, especially for up-regulation, during pollen germination and tube growth. In particular, genes of the calmodulin/calmodulin-like protein, cation/hydrogen exchanger, and heat shock protein families showed the most significant changes during pollen germination and tube growth. These results demonstrate that the overall transcription of genes, both in the number of expressed genes and in the levels of transcription, was increased. Furthermore, the appearance of many novel transcripts during pollen germination as well as tube growth indicates that these newly expressed genes may function in this complex process.
1 This work was supported by a competitive research grant for creative research groups sponsored by the National Science Foundation of China (grant no. 30421002).
2 These authors contributed equally to the article.
