日本一個研究小組日前在DNA Research雜志網(wǎng)絡(luò)版上報告說,,他們破譯了有“生物柴油樹”之稱的麻風(fēng)樹的基因組。
日本千葉縣上總DNA研究所,、國立遺傳學(xué)研究所和大阪大學(xué)組成的研究小組把麻風(fēng)樹的基因組分成小片段,,分析DNA序列,。研究人員破譯得出,,麻風(fēng)樹基因組共包含約4萬個基因,并且擁有許多與脂質(zhì)合成及抗病相關(guān)的基因,。研究人員認為,,這些都是麻風(fēng)樹不斷適應(yīng)干燥環(huán)境,,最終獲得的性質(zhì)。
麻風(fēng)樹別名南洋油桐,,廣泛分布于亞熱帶及干熱河谷地區(qū),,其果實有毒,不能食用,,但含油率高達60%至70%,,是一種非常有潛力的油料作物。經(jīng)改性后的麻風(fēng)樹油可適用于各種柴油發(fā)動機,。(生物谷Bioon.com)
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DNA Res (2011) 18 (1): 65-76. doi: 10.1093/dnares/dsq030
Sequence Analysis of the Genome of an Oil-Bearing Tree, Jatropha curcas L.
Shusei Sato1, Hideki Hirakawa1, Sachiko Isobe1, Eigo Fukai1, Akiko Watanabe1, Midori Kato1, Kumiko Kawashima1, Chiharu Minami1, Akiko Muraki1, Naomi Nakazaki1, Chika Takahashi1, Shinobu Nakayama1, Yoshie Kishida1, Mitsuyo Kohara1, Manabu Yamada1, Hisano Tsuruoka1, Shigemi Sasamoto1, Satoshi Tabata1,*, Tomoyuki Aizu2, Atsushi Toyoda2, Tadasu Shin-i2, Yohei Minakuchi2, Yuji Kohara2, Asao Fujiyama2,3, Suguru Tsuchimoto4, Shin'ichiro Kajiyama5, Eri Makigano6, Nobuko Ohmido6, Nakako Shibagaki7, Joyce A. Cartagena7, Naoki Wada7, Tsutomu Kohinata8, Alipour Atefeh8, Shota Yuasa8, Sachihiro Matsunaga8 and Kiichi Fukui8,*
Abstract
The whole genome of Jatropha curcas was sequenced, using a combination of the conventional Sanger method and new-generation multiplex sequencing methods. Total length of the non-redundant sequences thus obtained was 285 858 490 bp consisting of 120 586 contigs and 29 831 singlets. They accounted for ~95% of the gene-containing regions with the average G + C content was 34.3%. A total of 40 929 complete and partial structures of protein encoding genes have been deduced. Comparison with genes of other plant species indicated that 1529 (4%) of the putative protein-encoding genes are specific to the Euphorbiaceae family. A high degree of microsynteny was observed with the genome of castor bean and, to a lesser extent, with those of soybean and Arabidopsis thaliana. In parallel with genome sequencing, cDNAs derived from leaf and callus tissues were subjected to pyrosequencing, and a total of 21 225 unigene data have been generated. Polymorphism analysis using microsatellite markers developed from the genomic sequence data obtained was performed with 12 J. curcas lines collected from various parts of the world to estimate their genetic diversity. The genomic sequence and accompanying information presented here are expected to serve as valuable resources for the acceleration of fundamental and applied research with J. curcas, especially in the fields of environment-related research such as biofuel production. Further information on the genomic sequences and DNA markers is available at http://www.kazusa.or.jp/jatropha/.