中科院植物研究所系統(tǒng)與進(jìn)化植物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室陳之端研究組利用四個(gè)DNA片段（rbcL、matK,、trnL-F和26S rDNA）和65個(gè)廣義形態(tài)學(xué)形狀重建毛茛目的系統(tǒng)發(fā)育關(guān)系,，并更新了毛茛目的分類系統(tǒng)。該成果發(fā)表在《植物生態(tài)學(xué),、進(jìn)化與分類學(xué)展望》（Perspectives in Plant Ecology, Evolution and Systematics (2009, 11: 81-110)）雜志上,。
毛茛目(Ranunculales) 是真雙子葉植物最基部的分支之一，在被子植物大類群演化中起著承前啟后的作用,，系統(tǒng)位置極為關(guān)鍵,。通過選取毛茛目7科99屬植物，利用最大簡(jiǎn)約法和貝葉斯法,，在屬的水平上構(gòu)建了毛茛目的系統(tǒng)發(fā)育關(guān)系。根據(jù)所建立的高置信度的系統(tǒng)發(fā)育框架,，重新界定了毛茛目各科及科內(nèi)亞科或族,；探討了不同分類階元的鑒別性性狀；澄清了目?jī)?nèi)一些孤立屬的系統(tǒng)位置和分類等級(jí),。綜合分子和形態(tài)學(xué)證據(jù),，更新了毛茛目的分類系統(tǒng)，包括把防己科劃分為兩個(gè)新的亞科（Menispermoideae and Tinosporoideae),，為毛茛科的美花草屬(Callianthemum)建立了一新族Callianthemeae,。
該研究成果對(duì)我們理解毛茛目進(jìn)化的大格局和進(jìn)化的脈絡(luò)，以及理解整個(gè)被子植物,，特別是真雙子葉植物的多樣性和花部式樣的演化規(guī)律有重要意義,。（生物谷Bioon.com）
生物谷推薦原始出處：
Perspectives in Plant Ecology, Evolution and Systematics Volume 11, Issue 2, 2009, Pages 81-110
Phylogeny and classification of Ranunculales: Evidence from four molecular loci and morphological data
Wei Wanga, An-Ming Lua, Yi Renb, Mary E. Endressc and Zhi-Duan Chena, ,
aState Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Beijing 100093, PR China
bKey Laboratory of Medicinal Plant Resource and Natural Pharmaceutical Chemistry of Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi’an 710062, PR China
cInstitute of Systematic Botany, University of Zurich, Zollikerstrasse 107, 8008 Zurich, Switzerland
Abstract
Previous phylogenetic analyses of Ranunculales, which have mostly been focused on an individual family and were based on molecular data alone, have recovered three main clades within the order. However, support for relationships among these three clades was weak. Earlier hypotheses were often hampered by limited taxon sampling; to date less than one-tenth of the genera in the order have been sampled. In this study, we used a greatly enlarged taxon sampling (105 species, representing 99 genera of all seven families in the order). Our study is, furthermore, the first to employ morphology (65 characters) in combination with sequence data from four genomic regions, including plastid rbcL, matK and trnL-F, and nuclear ribosomal 26S rDNA to reconstruct phylogenetic relationships within Ranunculales. Maximum parsimony and Bayesian inference were performed on the individual and combined data sets. Our analyses concur with those of previous studies, but in most cases provide stronger support and better resolution for relationships among the three main clades retrieved. The first, comprised solely of the monogeneric family Eupteleaceae, is the earliest-diverging lineage. The second clade is composed exclusively of taxa of Papaveraceae, which is sister to the third clade, the core Ranunculales, comprising the other five families of the order. Circaeasteraceae and Lardizabalaceae form a strongly supported clade. Pteridophyllum is supported as sister to Hypecoum, contradicting the viewpoint that the former is the earliest-diverging genus in Papaveraceae. Glaucidium is basalmost in Ranunculaceae. Within this phylogenetic framework, the evolution of selected characters is inferred and diagnostic morphological characters at different taxonomic levels are identified and discussed. Based on both morphological and molecular evidence, a classification outline for Ranunculales is presented, including the proposal of two new subfamilies, Menispermoideae and Tinosporoideae in Menispermaceae and a new tribe, Callianthemeae, for the genus Callianthemum (Ranunculaceae).
