生物谷：科羅拉多州立大學（Colorado State University）的新研究顯示,，哺乳動物中所有基因的功能都是受晝夜節(jié)律（circadian rhythm；或者說日節(jié)律）調(diào)控的,。此項發(fā)表在6月15日PLoS Computational Biology雜志上的文章,，駁斥了現(xiàn)有理論，即僅僅10%～15%的基因受到生物鐘的影響,。盡管在此之前,，科學家們已經(jīng)認識到晝夜節(jié)律調(diào)控著生物體的行為，但是此項研究將之擺在了更為重要的地位,，即日節(jié)律可以掌控所有的生命功能,，尤其是代謝活動。此項研究認為振蕩（oscillation）是生物體所有基因的基本特征,，而非之前認為的只是部分基因的特殊功能,。
對于在代謝活動中相關(guān)基因的振蕩特性的了解，有助于我們對基因如何相互作用并調(diào)控健康和疾病的理解,。科羅拉多州立大學的研究者Andrey Ptitsyn搜集了相關(guān)研究,，并通過高級計算機算法為98%～99%的基因建立了基線,。在之前的研究結(jié)果中，當中的大部分基因從未有過與日周期相聯(lián)系的表達水平變化的報道,。甚至于一些基因被認為是持家基因（housekeeping gene）,，作為基因表達研究的穩(wěn)定照。
“只要正常進食的人都有體會,，人是不能太遲進食的,，現(xiàn)在我們離這個現(xiàn)象的答案更近一步了，” 獸醫(yī)和生物醫(yī)學學院（College of Veterinary Medicine and Biomedical Sciences）的研究員Ptitsyn介紹說,，“我們發(fā)現(xiàn)所有的基因在一天過程中總是呈現(xiàn)出顯著的變化規(guī)律,，這種規(guī)律體現(xiàn)在了活性的模式，或者表達的模式上,。每條基因表達通路都受到晝夜節(jié)律的影響,，而且不同通路基因的節(jié)律的同步性對于生命活動是非常重要的。”
（援引生命經(jīng)緯）
原始出處:
PLoS Computational Biology Vol. 3, No. 6, e120 doi:10.1371/journal.pcbi.0030120
Digital Signal Processing Reveals Circadian Baseline Oscillation in Majority of Mammalian Genes
Andrey A. Ptitsyn1*, Sanjin Zvonic2, Jeffrey M. Gimble2,3
1 Department of Microbiology, Immunology and Pathology, College of Veterinary and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, United States of America, 2 Stem Cell Laboratory, Clinical Nutrition Research Unit, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, Louisiana, United States of America, 3 Cell Biology Core Facility, Clinical Nutrition Research Unit, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, Louisiana, United States of America
In mammals, circadian periodicity has been described for gene expression in the hypothalamus and multiple peripheral tissues. It is accepted that 10%–15% of all genes oscillate in a daily rhythm, regulated by an intrinsic molecular clock. Statistical analyses of periodicity are limited by the small size of datasets and high levels of stochastic noise. Here, we propose a new approach applying digital signal processing algorithms separately to each group of genes oscillating in the same phase. Combined with the statistical tests for periodicity, this method identifies circadian baseline oscillation in almost 100% of all expressed genes. Consequently, circadian oscillation in gene expression should be evaluated in any study related to biological pathways. Changes in gene expression caused by mutations or regulation of environmental factors (such as photic stimuli or feeding) should be considered in the context of changes in the amplitude and phase of genetic oscillations.
Funding. The authors received no specific funding for this study.
Competing interests. The authors have declared that no competing interests exist.
Editor: Roy Kishony, Harvard University, United States of America
Citation: Ptitsyn AA, Zvonic S, Gimble JM (2007) Digital Signal Processing Reveals Circadian Baseline Oscillation in Majority of Mammalian Genes. PLoS Comput Biol 3(6): e120 doi:10.1371/journal.pcbi.0030120
Received: November 14, 2006; Accepted: May 14, 2007; Published: June 15, 2007
Copyright: © 2007 Ptitsyn et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abbreviations: K-S, Kolmogorov-Smirnov
* To whom correspondence should be addressed. E-mail: [email protected]
英文全文鏈接：
http://compbiol.plosjournals.org/perlserv/?request=get-document&doi=10.1371/journal.pcbi.0030120
