日本理化研究所20日發(fā)表新聞公報(bào)說，該所研究人員與文部科學(xué)省項(xiàng)目小組通過大規(guī)模數(shù)據(jù)分析，找到一群控制細(xì)胞分化狀態(tài)的轉(zhuǎn)錄因子,，并解開了其中的具體機(jī)制。
公報(bào)說,，研究人員以白血病患者的人體免疫細(xì)胞株THP－1為研究對象,，借助先進(jìn)的基因測序技術(shù)，按細(xì)胞分化過程中不同時(shí)間段收集它們從原單核細(xì)胞分化成單核細(xì)胞過程中整個基因組的數(shù)據(jù),。研究人員通過計(jì)算機(jī)對收集到的相關(guān)數(shù)據(jù)進(jìn)行了解析,，結(jié)果發(fā)現(xiàn)30種轉(zhuǎn)錄因子支配著細(xì)胞的分化過程，并了解了這些轉(zhuǎn)錄因子間相互作用的網(wǎng)絡(luò),。
公報(bào)指出,，該研究成果將使研究人員通過這些轉(zhuǎn)錄因子控制細(xì)胞分化狀態(tài)成為可能。（生物谷Bioon.com）
生物谷推薦原始出處：
Nature Genetics 19 April 2009 | doi:10.1038/ng.375
The transcriptional network that controls growth arrest and differentiation in a human myeloid leukemia cell line
The FANTOM Consortium, Riken Omics Science Center1 &
Using deep sequencing (deepCAGE), the FANTOM4 study measured the genome-wide dynamics of transcription-start-site usage in the human monocytic cell line THP-1 throughout a time course of growth arrest and differentiation. Modeling the expression dynamics in terms of predicted cis-regulatory sites, we identified the key transcription regulators, their time-dependent activities and target genes. Systematic siRNA knockdown of 52 transcription factors confirmed the roles of individual factors in the regulatory network. Our results indicate that cellular states are constrained by complex networks involving both positive and negative regulatory interactions among substantial numbers of transcription factors and that no single transcription factor is both necessary and sufficient to drive the differentiation process.
