來自瑞典哥特堡大學的研究人員首次識別了12種與侵略性乳癌相關的基因,。這項發(fā)現將有助于開發(fā)更可靠的疾病預測和治療方法,。
研究結果發(fā)布在Clinical Cancer Research雜志上，是基于97位乳癌患者得出的結論,。在這些患者中,，將近一般的患者在診斷出乳癌后的8年內已經死亡，而其他患者都存活超過8年,。
乳癌由多種不同的腫瘤細胞構成的,，從基因和生物特性上來說，這些細胞具有顯著差異性,。研究人員使用微陣列技術分析每個腫瘤中DNA和基因產物（RNA）的量,，以探究遺傳改變和臨床特征之間的關系，比如腫瘤性狀和對療法的應答,。
"我們已經成功識別了12個基因,，這些基因的表達與侵略性乳癌有關。"腫瘤學系在讀博士Toshima Parris表示,，"相對于那些還存活的患者,，這12個基因在那些已經死亡的患者中表達十分顯著。"
另外,，在侵略性乳癌中,，3個基因的產物表現出更高的水平。而剩下的9個基因在侵略性乳癌中表達水平更低,。
據Parris介紹,，這些基因會影響細胞生長，運動,，發(fā)育,，從而影響腫瘤的發(fā)展。在未來或能通過含有腫瘤細胞的血液樣本測試這些標記,，確定是否患者能從特定的療法或藥物中獲益,。（生物谷Bioon.com）
【生物谷會議推薦：
2010細胞治療研究進展與臨床應用前沿研討會 2010.09.23-2010.09.25
會議官方網址：www.Cell-therapies.net 】
生物谷推薦原始出處：
Clinical Cancer Research, DOI: 10.1158/1078-0432.CCR-10-0889
Expand+Clinical Implications of Gene Dosage and Gene Expression Patterns in Diploid Breast Carcinoma
Toshima Z. Parris1, Anna Danielsson1, Szilárd Nemes1, Anikó Kovács2, Ulla Delle1, Ghita Fallenius1, Elin M?llerstr?m1, Per Karlsson1, and Khalil Helou1
Purpose: Deregulation of key cellular pathways is fundamental for the survival and expansion of neoplastic cells. In cancer, regulation of gene transcription can be mediated in a variety of ways. The purpose of this study was to assess the impact of gene dosage on gene expression patterns and the effect of other mechanisms on transcriptional levels, and to associate these genomic changes with clinicopathologic parameters.
Experimental Design: We screened 97 invasive diploid breast tumors for DNA copy number alterations and changes in transcriptional levels using array comparative genomic hybridization and expression microarrays, respectively.
Results: The integrative analysis identified an increase in the overall number of genetic alterations during tumor progression and 15 specific genomic regions with aberrant DNA copy numbers in at least 25% of the patient population, i.e., 1q22, 1q22-q23.1, 1q25.3, 1q32.1, 1q32.1-q32.2, 8q21.2-q21.3, 8q22.3, 8q24.3, and 16p11.2 were recurrently gained, whereas 11q25, 16q21, 16q23.3, and 17p12 were frequently lost (P < 0.01). An examination of the expression patterns of genes mapping within the detected genetic aberrations identified 47 unique genes and 1 Unigene cluster significantly correlated between the DNA and relative mRNA levels. In addition, more malignant tumors with normal gene dosage levels displayed a recurrent overexpression of UBE2C, S100A8, and CBX2, and downregulation of LOC389033, STC2, DNALI1, SCUBE2, NME5, SUSD3, SERPINA11, AZGP1, and PIP.
Conclusions: Taken together, our findings suggest that the dysregulated genes identified here are critical for breast cancer initiation and progression, and could be used as novel therapeutic targets for drug development to complement classical clinicopathologic features. Clin Cancer Res; 16(15); 3860–74. ?2010 AACR.
