生物谷報(bào)道：3 種核蛋白MINT 〔也叫X11樣（X11-like）蛋白質(zhì)〕是多突觸蛋白質(zhì)的結(jié)合物，最近的一項(xiàng)研究利用淘汰法證明這3 種核蛋白MINT可能在突觸傳遞中不可或缺,。該研究結(jié)果發(fā)表在12月13日的《Journal of Neuroscience》上,。
由于不同的異構(gòu)體（isoform）能相互彌補(bǔ)彼此的功能不足；因此,，應(yīng)用單淘汰法較難得出確實(shí)可靠的結(jié)論,。因此,，安琪拉·何（Angela Ho）等人應(yīng)用構(gòu)成條件淘汰法（constitutive and conditional knock-out strategies）剪切3 種核蛋白MINT,。核蛋白MINT 1和2的剪切使這兩種蛋白的異構(gòu)體選擇神經(jīng)元為特定表達(dá)對(duì)象，導(dǎo)致大多數(shù)老鼠在出生時(shí)死亡。幸存的20%小鼠有共濟(jì)失調(diào)并且體重降低,。在雙淘汰法研究中,，海馬神經(jīng)元的全細(xì)胞記錄顯示突觸強(qiáng)度降低，微興奮性突觸后電流(excitatory postsynaptic currents, EPSCs)的頻率縮減了兩倍,，對(duì)脈沖易化（paired pulse facilitation, PPF）提高,，核蛋白MINT1和2提示有突觸前活動(dòng)。 相似結(jié)果見于核蛋白MINT1/2/3的急性脫落(消融)試驗(yàn),。
原文出處：
Angela Ho, Wade Morishita, Deniz Atasoy, Xinran Liu, Katsuhiko Tabuchi, Robert E. Hammer, Robert C. Malenka, and Thomas C. Südhof
Genetic Analysis of Mint/X11 Proteins: Essential Presynaptic Functions of a Neuronal Adaptor Protein Family
J. Neurosci. 2006 26: 13089-13101; doi:10.1523/JNEUROSCI.2855-06.2006 [Abstract] [Full Text] [PDF] [Supplemental Data]
背景知識(shí)：
MINT（Msx2 interacting nuclear target protein）是定位于細(xì)胞核基質(zhì)中的一種蛋白質(zhì),，廣泛分布于成體的多種組織，對(duì)轉(zhuǎn)錄有負(fù)調(diào)控作用. MINT是通過對(duì)同源轉(zhuǎn)錄抑制因子Msx2為誘餌的相互作用蛋白的篩選而被發(fā)現(xiàn)的
相關(guān)基因：
X11L
X11L [Drosophila melanogaster]
Other Aliases: Dmel_CG5675, CG5675, CG5678, Dmint, MINT, X11, dX11L
Other Designations: X11L CG5675-PA
Chromosome: X; Location: 16B7-16B7
GeneID: 252671
Msx2
Official Symbol: Msx2 and Name: homeo box, msh-like 2 [Mus musculus]
Other Aliases: BB122635, Hox-8, Hox8, Hox8.1
Chromosome: 13; Location: 13 32.0 cM
GeneID: 17702
作者簡(jiǎn)介：
Angela Ho
Research Interests
I have diverse interests.
They can be broadly categorized to include Human Factors and Human-Computer Interaction, applicable to Command and Control domains like in vehicles and aircrafts.I am also interested in applying engineering knowledge to the greater fields of Strategies, Policy Making in the Transportation, Aviation and Defence industries.
Research Advisor Prof. Mary (Missy) Cummings
Department of Aeronautics and Astronautics
