生物谷報道：根據(jù)布朗大學(xué)發(fā)表的一篇新研究顯示,，在三維空間培養(yǎng)的神經(jīng)細胞，與傳統(tǒng)的平面培養(yǎng)皿培養(yǎng)的神經(jīng)細胞,，有1,766 個基因的表現(xiàn)不同,。
這項研究也發(fā)現(xiàn)，在三維空間培養(yǎng)的生長神經(jīng)細胞,，與人體中的生長情況更相似,。因此培養(yǎng)方法是相當(dāng)重要的。如果想要更了解細胞在人體中的基因表現(xiàn)或新藥物如何與疾病戰(zhàn)斗,，三維空間的培養(yǎng)是更理想的,。
科學(xué)家在平面的培養(yǎng)皿中培養(yǎng)細胞已經(jīng)超過100年了，細胞黏附于培養(yǎng)皿底部,，當(dāng)增生時便蔓延開來,。但是細胞在身體中的生長并非如此。它們懸浮于液體和膠體中,，并且被其它細胞圍繞著,。而且這些細胞并不是固定的，它們是會移動的,。
所以有些科學(xué)家懷疑,，培養(yǎng)皿中的細胞表現(xiàn)并不如活體中的情況。這意味著,，科學(xué)家在培養(yǎng)皿中了解到的細胞功能,，可能與實際情況有所偏差。
這項研究發(fā)表于5月號的Tissue Engineering中,，研究結(jié)果顯示,，培養(yǎng)技術(shù)可能對于細胞生長和功能有顯著的影響。
(資料來源 : biocompare)
英文原文鏈接：
http://news.biocompare.com/newsstory.asp?id=183078
原始出處:
Tissue Engineering
Genomic and Morphological Changes of Neuroblastoma Cells in Response to Three-Dimensional Matrices
May 2007, Vol. 13, No. 5 : 1035 -1047
Grace N. Li, M.Eng.
From the Center for Biomedical Engineering, Department of Molecular Pharmacology, Physiology, and Biotechnology Brown University, Providence, Rhode Island.
Liane L. Livi, M.S.
From the Center for Biomedical Engineering, Department of Molecular Pharmacology, Physiology, and Biotechnology Brown University, Providence, Rhode Island.
Celinda M. Gourd, B.S.
From the Center for Biomedical Engineering, Department of Molecular Pharmacology, Physiology, and Biotechnology Brown University, Providence, Rhode Island.
Elizabeth S. Deweerd, M.S.
From the Center for Biomedical Engineering, Department of Molecular Pharmacology, Physiology, and Biotechnology Brown University, Providence, Rhode Island.
Diane Hoffman-Kim, Ph.D.
From the Center for Biomedical Engineering, Department of Molecular Pharmacology, Physiology, and Biotechnology Brown University, Providence, Rhode Island.
Advances in neural tissue engineering require a comprehensive understanding of neuronal growth in 3 dimensions. This study compared the gene expression of SH-SY5Y human neuroblastoma cells cultured in 3-dimensional (3D) with those cultured in 2-dimensional (2D) environments. Microarray analysis demonstrated that, in response to varying matrix geometry, SH-SY5Y cells exhibited differential expression of 1,766 genes in collagen I, including those relevant to cytoskeleton, extracellular matrix, and neurite outgrowth. Cells extended longer neurites in 3D collagen I cultures than in 2D. Real-time reverse transcriptase polymerase chain reaction experiments and morphological analysis comparing collagen I and Matrigel tested whether the differential growth and gene expression reflected influences of culture dimension or culture material. SH-SY5Y neuroblastoma cells responded to geometry by differentially regulating cell spreading and genes associated with actin in similar patterns for both materials; however, neurite outgrowth and the expression of the gene encoding for neurofilament varied with the type of material. Electron microscopy and mechanical analysis showed that collagen I was more fibrillar than Matrigel, with larger inter-fiber distance and higher stiffness. Taken together, these results suggest complex cell–material interactions, in which the dimension of the culture material influences gene expression and cell spreading and the structural and mechanical properties of the culture material influence gene expression and neurite outgrowth.
