日本慶應(yīng)義塾大學(xué)研究人員日前在動物實驗中發(fā)現(xiàn),，在老鼠小腦中,，有兩種蛋白質(zhì)的復(fù)合體能促進神經(jīng)細胞的黏結(jié)和成熟,。這一研究成果已刊登在新一期美國《科學(xué)》雜志上。
研究人員發(fā)現(xiàn),，在老鼠小腦的顆粒細胞和“Purkinje”細胞之間,，“Cbln1”和“GluD2”這兩種蛋白質(zhì)形成了復(fù)合體。這種復(fù)合體像“糨糊”一樣在細胞之間發(fā)揮作用,，促進了細胞的黏結(jié)和成熟,，對于形成和維持神經(jīng)回路發(fā)揮著重要作用。
研究人員說,，這一發(fā)現(xiàn)將有助于治療因小腦疾病而導(dǎo)致的運動障礙等,。(生物谷Bioon.com)
生物谷推薦原文出處：
Science DOI: 10.1126/science.1185152
Cbln1 Is a Ligand for an Orphan Glutamate Receptor 2, a Bidirectional Synapse Organizer
Keiko Matsuda,1 Eriko Miura,1 Taisuke Miyazaki,2 Wataru Kakegawa,1 Kyoichi Emi,1 Sakae Narumi,1 Yugo Fukazawa,3 Aya Ito-Ishida,1,4 Tetsuro Kondo,1,5 Ryuichi Shigemoto,3 Masahiko Watanabe,2 Michisuke Yuzaki1,*
Cbln1, secreted from cerebellar granule cells, and the orphan glutamate receptor 2 (GluD2), expressed by Purkinje cells, are essential for synapse integrity between these neurons in adult mice. Nevertheless, no endogenous binding partners for these molecules have been identified. We found that Cbln1 binds directly to the N-terminal domain of GluD2. GluD2 expression by postsynaptic cells, combined with exogenously applied Cbln1, was necessary and sufficient to induce new synapses in vitro and in the adult cerebellum in vivo. Further, beads coated with recombinant Cbln1 directly induced presynaptic differentiation and indirectly caused clustering of postsynaptic molecules via GluD2. These results indicate that the Cbln1-GluD2 complex is a unique synapse organizer that acts bidirectionally on both pre- and postsynaptic components.
1 Department of Physiology, School of Medicine, Keio University, Tokyo 160-8582, Japan.
2 Department of Anatomy, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan.
3 Division of Cerebral Structures, National Institutes for Physiological Sciences, Okazaki 444-8787, Japan.
4 Department of Cellular Neurobiology, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan.
5 Molecular Neurophysiology, Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan.
