德國(guó)學(xué)者近日在《神經(jīng)病學(xué)文獻(xiàn)》（Archives of Neurology）雜志報(bào)道稱,，彌散性神經(jīng)膠質(zhì)瘤不應(yīng)當(dāng)被作為病灶性疾病對(duì)待,，而應(yīng)該被看作一種涉及整個(gè)大腦的系統(tǒng)性疾病,。
在一項(xiàng)包括4名彌散性神經(jīng)膠質(zhì)瘤患者的研究中,，研究人員發(fā)現(xiàn)在大腦一些不易引起注意的區(qū)域中可以檢測(cè)到單個(gè)的腫瘤細(xì)胞,，組織學(xué)分析發(fā)現(xiàn)這些細(xì)胞具有多形性及分裂活性,。
研究者稱,，檢測(cè)彌散性神經(jīng)膠質(zhì)瘤的浸潤(rùn)范圍長(zhǎng)期以來(lái)都是個(gè)困擾神經(jīng)病學(xué)家的難題,，因?yàn)橐袁F(xiàn)有技術(shù)，單個(gè)的神經(jīng)膠質(zhì)瘤細(xì)胞很難被檢測(cè)出來(lái),。
最近有研究表明,，在多數(shù)彌散性神經(jīng)膠質(zhì)瘤細(xì)胞中存在編碼異檸檬酸脫氫酶1（IDH1）的基因變異，IDH1變異可引起其編碼蛋白質(zhì)第132位（R132H）上的精氨酸殘基置換為組氨酸殘基,，另外一種單克隆抗體H09可識(shí)別R132H突變,。該途徑可用于檢測(cè)單個(gè)腫瘤細(xì)胞。
在該研究中,，研究者使用該抗體檢測(cè)了4名已知有IDH1 R132H突變神經(jīng)膠質(zhì)瘤患者的腦組織,。發(fā)現(xiàn)大腦中多個(gè)區(qū)域均可檢測(cè)到神經(jīng)膠質(zhì)瘤細(xì)胞,。
作者稱，這種新型IDH1突變和IHC免疫組化方法對(duì)有IDH1突變的神經(jīng)膠質(zhì)瘤細(xì)胞進(jìn)行原位檢測(cè),，將對(duì)大腦腫瘤的診斷產(chǎn)生重大影響,。（生物谷bioon.com）
doi:10.1001/archneurol.2011.2910
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Addressing Diffuse Glioma as a Systemic Brain Disease With Single-Cell Analysis
Felix Sahm, MD; David Capper, MD; Astrid Jeibmann, MD; Antje Habel; Werner Paulus, MD; Dirk Troost, MD; Andreas von Deimling, MD.
Objective To analyze infiltration patterns of IDH1 mutant diffuse gliomas into the brain by identification of single tumor cells applying an antibody specific to mutant IDH1 R132H protein.
Design Immunohistochemical analysis.
Setting University hospital.
Patients Whole-brain and hemisphere sections of 4 patients diagnosed with diffuse glioma.
Results Tumor cells were identified in areas that appeared inconspicuous macroscopically and at histological analysis with respect to cellularity, cellular pleomorphism, or mitotic activity in all cases.
Conclusion Detection of single tumor cells throughout the brain demonstrates diffuse glioma to represent systemic brain disease.
