成年魚類大腦有大量放射狀膠質(zhì)細胞,，直至成年期，但放射狀膠質(zhì)細胞在成年動物神經(jīng)發(fā)生過程中的作用尚不明了,。課題組的前期研究表明,，櫻花鉤吻鮭與其他魚種不同的是，酪氨酸羥化酶免疫定位第三腦室和大腦尾區(qū)的長放射狀突起的細胞中,。發(fā)表在2013年1月第1期《中國神經(jīng)再生研究（英文版）》雜志的“Features of adult neurogenesis and neurochemical signaling in the Cherry salmon Oncorhynchus masou brain”的研究表明,，在櫻花鉤吻鮭生后不同發(fā)育階段，室周細胞表現(xiàn)出神經(jīng)元和膠質(zhì)細胞,，包括放射狀膠質(zhì)細胞的形態(tài),，并包含多種神經(jīng)化學物質(zhì)。大腦腳背蓋背內(nèi)側(cè),、髓質(zhì)和脊髓中央灰質(zhì)層,、間腦室周圍區(qū)的細胞增殖區(qū)出現(xiàn)酪氨酸羥化酶、γ氨基丁酸和NADPH黃遞酶免疫陽性細胞。Pax6免疫定位檢測揭示鮭魚在生后不同發(fā)育階段腦結(jié)構(gòu)變化,，這些變化也得到酪氨酸羥化酶和γ氨基丁酸免疫組化證實,。(生物谷Bioon.com)
doi:10.3969/j.issn.1673-5374.2013.01.002
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Features of adult neurogenesis and neurochemical signaling in the Cherry salmon Oncorhynchus masou brain
Evgeniya V. Pushchina1, Dmitry K. Оbukhov2, Anatoly A. Varaksin1
We investigated the distribution of gamma aminobutyric acid, tyrosine hydroxylase and nitric oxide-producing elements in a cherry salmon Oncorhynchus masou brain at various stages of postnatal ontogenesis by immunohistochemical staining and histochemical staining. The periventricular region cells exhibited the morphology of neurons and glia including radial glia-like cells and contained several neurochemical substances. Heterogeneous populations of tyrosine hydroxylase-, gamma aminobutyric acid-immunoreactive, аs well as nicotinamide adenine dinucleotide phosphate diaphorase-positive cells were observed in proliferating cell nuclear antigen-immunoreactive proliferative zones in periventricular area of diencephalon, central grey layer of dorsomedial tegmentum, medulla and spinal cord. Immunolocalization of Pax6 in the cherry salmon brain revealed a neuromeric construction of the brain at various stages of postnatal ontogenesis, and this was confirmed by tyrosine hydroxylase and gamma aminobutyric acid labeling.
