神經(jīng)示蹤作為一種神經(jīng)解剖學(xué)研究的常用技術(shù)，有助于研究神經(jīng)環(huán)路和軸突投射,，周圍神經(jīng)損傷后的再生以及脊髓損傷后傳導(dǎo)束的重建。熒光示蹤劑可直接顯示標(biāo)記的神經(jīng)元，無(wú)需后期染色,，具備有：可特異性標(biāo)記神經(jīng)元，標(biāo)記效率高,，不易淬滅,，可被快速運(yùn)輸?shù)桨胁课患暗投拘缘葍?yōu)點(diǎn)。
但目前能完全滿足上述要求的熒光示蹤劑并不多,。蘇州大學(xué)顧劍輝教授所在課題組進(jìn)行的一項(xiàng)關(guān)于“Neurological function following intra-neural injection of fluorescent neuronal tracers in rats”的研究,，建立了大鼠脛神經(jīng)注射示蹤劑模型，評(píng)價(jià)3種示蹤劑Fluoro-Gold、True blue和Fluoro-Ruby對(duì)大鼠神經(jīng)功能的影響,。
結(jié)果證實(shí),，大鼠脛神經(jīng)內(nèi)注射Fluoro-Gold示蹤劑可顯著抑制運(yùn)動(dòng)、感覺(jué)和植物神經(jīng)功能,，注射Fluoro-Gold示蹤劑對(duì)神經(jīng)功能影響最嚴(yán)重,，注射True blue示蹤劑次之，而注射Fluoro-Ruby示蹤劑基本對(duì)神經(jīng)功能無(wú)影響,。作者認(rèn)為,，文章結(jié)果是國(guó)際上從神經(jīng)功能角度探討示蹤劑毒性的首次報(bào)道，可為未來(lái)研究神經(jīng)重支配準(zhǔn)確性提供理論基礎(chǔ),。
文章發(fā)表在《中國(guó)神經(jīng)再生研究（英文版）》2013年5月第14期的雜志上,。（生物谷Bioon.com）
doi:10.3969/j.issn.1673-5374.2013.14.001
PMC:
PMID:
Neurological function following intra-neural injection of fluorescent neuronal tracers in rats
Wen Hu1, 2, Dan Liu2, 3, Yanping Zhang2, 3, Zhongyi Shen2, 4, Tianwen Gu2, 4, Xiaosong Gu1, 2, Jianhui Gu3
Fluorescent neuronal tracers should not be toxic to the nervous system when used in long-term labeling. Previous studies have addressed tracer toxicity, but whether tracers injected into an intact nerve result in functional impairment remains to be elucidated. In the present study, we examined the functions of motor, sensory and autonomic nerves following the application of 5% Fluoro-Gold, 4% True Blue and 10% Fluoro-Ruby (5 μL) to rat tibial nerves via pressure injection. A set of evaluation methods including walking track analysis, plantar test and laser Doppler perfusion imaging was used to determine the action of the fluorescent neuronal tracers. Additionally, nerve pathology and ratio of muscle wet weight were also observed. Results showed that injection of Fluoro-Gold significantly resulted in loss of motor nerve function, lower plantar sensibility, increasing blood flow volume and higher neurogenic vasodilatation. Myelinated nerve fiber degeneration, unclear boundaries in nerve fibers and high retrograde labeling efficacy were observed in the Fluoro-Gold group. The True Blue group also showed obvious neurogenic vasodilatation, but less severe loss of motor function and degeneration, and fewer labeled motor neurons were found compared with the Fluoro-Gold group. No anomalies of motor and sensory nerve function and no myelinated nerve fiber degeneration were observed in the Fluoro-Ruby group. Experimental findings indicate that Fluoro-Gold tracing could lead to significant functional impairment of motor, sensory and autonomic nerves, while functional impairment was less severe following True Blue tracing. Fluoro-Ruby injection appears to have no effect on neurological function.
