植物同人類一樣具有識(shí)別病原微生物并激發(fā)免疫反應(yīng)的能力。認(rèn)識(shí)其中的關(guān)鍵機(jī)理對(duì)改良農(nóng)作物抗病,、保障糧食生產(chǎn)安全具有重要意義,。
中科院遺傳與發(fā)育生物研究所的周儉民實(shí)驗(yàn)室通過與清華大學(xué)的柴繼杰實(shí)驗(yàn)室和英國Sainsbury Laboratory的Cyril Zipfel實(shí)驗(yàn)室密切合作，揭示了植物的重要免疫受體FLS2識(shí)別細(xì)菌運(yùn)動(dòng)所必需的鞭毛蛋白的機(jī)理,。鞭毛蛋白在植物細(xì)胞外直接結(jié)合FLS2并招募共同受體BAK1,，從而激活植物免疫系統(tǒng)。由于FLS2所代表的免疫受體識(shí)別病原微生物中廣泛存在的分子,，該研究的發(fā)現(xiàn)將有助于設(shè)計(jì)廣譜抗病的農(nóng)作物,。
這項(xiàng)工作于10月11日在《科學(xué)》雜志在線發(fā)表。清華大學(xué)的研究生孫亞東和遺傳發(fā)育所的研究生李磊是該文的共同第一作者,。
周儉民實(shí)驗(yàn)室的研究得到了中國科技部,、國家自然科學(xué)基金和中國科學(xué)院的資助（生物谷Bioon.com）,。
生物谷推薦的英文摘要
Science doi:10.1126/science.1243825
Structural Basis for flg22-Induced Activation of the Arabidopsis FLS2-BAK1 Immune Complex
Yadong Sun1，*,， Lei Li2,， Alberto P. Macho3， Zhifu Han1,，Zehan Hu1,， Cyril Zipfel3， Jian-min Zhou2,，,， Jijie Chai1
Flagellin perception in Arabidopsis is through recognition of its highly conserved N-terminal epitope (flg22) by flagellin-sensitive 2 (FLS2). Flg22 binding induces FLS2 heteromerization with BRI1-associated kinase 1 (BAK1) and their reciprocal activation followed by plant immunity. Here， we report the crystal structure of FLS2 and BAK1 ectodomains complexed with flg22 at 3.06 angstroms. A conserved and a nonconserved site from the inner surface of the FLS2 solenoid recognize the C- and N-terminal segment of flg22,， respectively,， without oligomerization or conformational changes in the FLS2 ectodomain. Besides directly interacting with FLS2， BAK1 acts as a coreceptor by recognizing the C terminus of the FLS2-bound flg22. Our data reveal the molecular mechanisms underlying FLS2-BAK1 complex recognition of flg22 and provide insight into the immune receptor complex activation.
