英國鄧迪大學(xué)研究人員通過分析大腸桿菌基因組新發(fā)現(xiàn)三個(gè)大腸桿菌生長發(fā)育必需基因，其中一個(gè)基因也在人體中發(fā)現(xiàn),，與癌癥發(fā)生密切相關(guān),。
研究人員將新發(fā)現(xiàn)的三個(gè)基因命名為：yjeE, yeaZ和ygjD,。實(shí)驗(yàn)證明三個(gè)基因與大腸桿菌正常生長發(fā)育密切相關(guān),，限制yeaZ基因表達(dá)會(huì)使大腸桿菌細(xì)胞核區(qū)高度濃縮,，限制yjeE和ygjD基因表達(dá)則導(dǎo)致DNA在細(xì)胞中分配不均。
研究人員還發(fā)現(xiàn)ygjD基因也出現(xiàn)在人類基因組中,，是新發(fā)現(xiàn)的三個(gè)基因中最重要的一個(gè),。（生物谷Bioon.com）
生物谷推薦原始出處：
Journal of Bacteriology, August 2009,doi:10.1128/JB.00136-09
Conserved Network of Proteins Essential for Bacterial Viability ,
Jennifer I. Handford,1, Bérengère Ize,1,2,, Grant Buchanan,3 Gareth P. Butland,4,5 Jack Greenblatt,5 Andrew Emili,5 and Tracy Palmer3*
Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich NR4 7UH, United Kingdom,1 School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom,2 Division of Molecular and Environmental Microbiology, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom,3 Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California, 94720,4 Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada M5S 3E15
The yjeE, yeaZ, and ygjD genes are highly conserved in the genomes of eubacteria, and ygjD orthologs are also found throughout the Archaea and eukaryotes. In this study, we have constructed conditional expression strains for each of these genes in the model organism Escherichia coli K12. We show that each gene is essential for the viability of E. coli under laboratory growth conditions. Growth of the conditional strains under nonpermissive conditions results in dramatic changes in cell ultrastructure. Deliberate repression of the expression of yeaZ results in cells with highly condensed nucleoids, while repression of yjeE and ygjD expression results in at least a proportion of very enlarged cells with an unusual peripheral distribution of DNA. Each of the three conditional expression strains can be complemented by multicopy clones harboring the rstA gene, which encodes a two-component-system response regulator, strongly suggesting that these proteins are involved in the same essential cellular pathway. The results of bacterial two-hybrid experiments show that YeaZ can interact with both YjeE and YgjD but that YgjD is the preferred interaction partner. The results of in vitro experiments indicate that YeaZ mediates the proteolysis of YgjD, suggesting that YeaZ and YjeE act as regulators to control the activity of this protein. Our results are consistent with these proteins forming a link between DNA metabolism and cell division.
