微生物學(xué)通報 20 June 2009, 36(6):931～935
一種雙歧桿菌胞外多糖免疫調(diào)節(jié)功能研究
李偉欣 陳 倩 李平蘭* 程 靜
(中國農(nóng)業(yè)大學(xué)食品科學(xué)與營養(yǎng)工程學(xué)院 北京 100083)
摘 要: 根據(jù)保健食品功能評價規(guī)范——功能學(xué)評價程序研究一種雙歧桿菌胞外多糖(Bifidobacterium spp. exopolysaccharide, EPS)的免疫調(diào)節(jié)功能,。通過脾淋巴細胞增殖反應(yīng),、綿羊紅細胞誘導(dǎo)小鼠遲發(fā)型變態(tài)反應(yīng)(DTH)、血清溶血素測定以及巨噬細胞吞噬實驗探討該EPS的免疫調(diào)節(jié)活性,。EPS分別以高,、中、低劑量組連續(xù)口服給藥10天, 結(jié)果發(fā)現(xiàn)低劑量的EPS[100 mg/(kg·d)]可以促進脾淋巴細胞增殖; 高,、中,、低劑量對綿羊紅細胞誘導(dǎo)的小鼠遲發(fā)型變態(tài)反應(yīng)均無促進作用; 但是, EPS 高、中,、低劑量組均能明顯提高小鼠血清半數(shù)溶血值HC50以及增強小鼠腹腔巨噬細胞吞噬雞紅細胞的吞噬能力,。根據(jù)規(guī)范可知, 該雙歧桿菌EPS 具有一定的免疫調(diào)節(jié)活性,。
關(guān)鍵詞: 雙歧桿菌, 胞外多糖, 免疫
Study on Immunoregulation Effect of Exopolysaccharide Produced by Bifidobacterium spp.
LI Wei-Xin CHEN Qian LI Ping-Lan* CHENG Jing
(Food Science and Nutrition Engineering College, China Agricultural University, Beijing 100083, China)
Abstract: The immunoregulation effect to polysaccharides from Bifidobacterium spp. was investigated on the base of functional assessment standards of health food. Effects of the EPS on immunity were investigated by promoted the proliferation of spleen lymph cells, delayed type hypersensitivity reaction, the HC50 value and macrophage function assay in mice. Data showed that the EPS could obviously increase the ratio of swallowed chicken red blood cell by macrophage and the HC50 value in mice. However, no significant effect was found on the delayed type hypersensitive induced by sheep red blood cell, for only the low dose of 100 mg/(kg·d) EPS promoted the proliferation of spleen lymph cells. Bifidobacterium spp. EPS can certain immunomodulating function.
Keywords: Bifidobacterium, Exopolysaccharide, Immunomodulating
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