微生物學(xué)通報(bào) 20 JUN 2009, 36(6):789～793
產(chǎn)L-絲氨酸菌株SYPS-062的鑒定及碳源對(duì)發(fā)酵的影響
張曉梅1 竇文芳1 許泓瑜1 許正宏1,2*
(1. 江南大學(xué)醫(yī)藥學(xué)院制藥工程研究室 江蘇 無(wú)錫 214122)
(2. 江南大學(xué)工業(yè)生物技術(shù)教育部重點(diǎn)實(shí)驗(yàn)室 江蘇 無(wú)錫 214122)
摘 要: 采用形態(tài)學(xué),、生理生化實(shí)驗(yàn)和16S rDNA序列分析的方法對(duì)從自然界中篩選得到的一株能直接利用糖質(zhì)原料發(fā)酵生產(chǎn)L-絲氨酸菌株SYPS-062的分類地位進(jìn)行了研究, 確定其為谷氨酸棒桿菌(Corynebacterium glutamicum)。同時(shí)考察了碳源對(duì)菌株SYPS-062發(fā)酵產(chǎn)L-絲氨酸的影響, 實(shí)驗(yàn)結(jié)果表明, 當(dāng)蔗糖濃度為60 g/L時(shí), 菌株SYPS-062生物量和L-絲氨酸的積累均達(dá)到最大值, 分別為8.1 g/L和6.6 g/L,。
關(guān)鍵詞: L-絲氨酸, 谷氨酸棒桿菌, 鑒定, 糖質(zhì)原料, 直接發(fā)酵
Identification of L-Serine Producer SYPS-062 and the Effect of Different Carbon Source
ZHANG Xiao-Mei1 DOU Wen-Fang1 XU Hong-Yu1 XU Zheng-Hong1,2*
(1. Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics,Jiangnan University, Wuxi, Jiangsu 214122, China)
(2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China)
Abstract: SYPS-062 was an L-serine producing strain stored by our lab, which could directly produce L-serine from sugar. According to morphology, physiological and biochemical identification and 16S rDNA sequence, SYPS-062 was identified as Corynebacterium glutamicum. And the effect of different carbon source for the SYPS-062 and Corynebacterium glutamicum ATCC13032 fermentation were studied. The experiment result showed that the sucrose was 60 g/L, the yield of L-serine and biomass reached the maximal value, 6.6 g/L and 8.1 g/L respectively.
Keywords: L-serine, Corynebacterium glutamicum, Identification, Sugar, Direct fermentation
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