生物工程學(xué)報 25 June 2009, 25(6):932～940
放線菌與枯草芽孢桿菌的共培養(yǎng)及其對活性次生代謝產(chǎn)物的影響
黃兵1,2, 劉寧2, 黃英2, 陳勁春1
1 北京化工大學(xué)生命科學(xué)與技術(shù)學(xué)院, 北京 100029
2 中國科學(xué)院微生物研究所 微生物資源前期開發(fā)國家重點實驗室, 北京 100101
摘 要: 為探討共培養(yǎng)對放線菌產(chǎn)生活性次生代謝產(chǎn)物的影響, 結(jié)合抗菌活性測定及HPLC-PDA分析, 研究了22株放線菌的單培養(yǎng)及其與枯草芽孢桿菌的共培養(yǎng)發(fā)酵代謝產(chǎn)物的差異, 并選取抗菌活性較強(qiáng)的鏈霉菌FXJ2.014進(jìn)一步研究其代謝產(chǎn)物,。發(fā)現(xiàn)FXJ2.014、FXJ1.296,、AS 4.1252三株菌與枯草芽孢桿菌共培養(yǎng)時產(chǎn)生其在相同條件下單培養(yǎng)時沒有的物質(zhì), 其中鏈霉菌FXJ2.014單培養(yǎng)時主要產(chǎn)生醌霉素A, 共培養(yǎng)時產(chǎn)物中增加了醌霉素結(jié)構(gòu)類似物FXJ2.014-HB,。進(jìn)一步的抗菌、抗腫瘤活性測定結(jié)果表明, 兩者的生物活性有較顯著的差異, 且FXJ2.014-HB對多種腫瘤細(xì)胞系的抑制活性普遍弱于高毒性的醌霉素A, 為有潛力的細(xì)胞毒性較小的抗生素,。共培養(yǎng)是一條很有希望的發(fā)掘放線菌活性次生代謝產(chǎn)物的新途徑,。
關(guān)鍵詞: 放線菌, 枯草芽孢桿菌, 共培養(yǎng), 鏈霉菌, 活性次生代謝產(chǎn)物, 醌霉素
Coculture of actinomycetes with Bacillus subtilis and its effect on the bioactive secondary metabolites
Bing Huang1,2, Ning Liu2, Ying Huang2, and Jinchun Chen1
1 College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
2 State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Abstract: To explore the effect of coculturing actinomycetes with Bacillus subtilis on the production of bioactive secondary metabolites, we studied the difference between fermentation products of monocultures and the corresponding cocultures of 22 actinomycetes by antimicrobial assay and HPLC-PDA analysis. We selected Streptomyces strain FXJ2.014 with high bioactivity for further analysis and found additional metabolites in fermentation extracts of cocultures of strains FXJ2.014, FXJ1.296 and AS 4.1252 respectively with B. subtilis. Quinomycin A was the main bioactive metabolite produced by the monoculture of strain FXJ2.014, while a new quinomycin-like component named FXJ2.014-HB was produced when strain FXJ2.014 was cocultured with B. subtilis. Further tests of antimicrobial and antitumor activities indicated that FXJ2.014-HB and Quinomycin A had significant differences in terms of bioactivity. Moreover, the inhibitory activity of FXJ2.014-HB to a variety of tumor cell lines was weaker than the highly toxic Quinomycin A, indicating its potential to be an antibiotic with low cell toxicity. In conclusion, coculture can be used as a promising approach to discover bioactive secondary metabolites from actinomycetes.
Keywords: actinomycetes, Bacillus subtilis, coculture, Streptomyces, bioactive secondary metabolites, quinomycin
生物谷推薦全文下載：放線菌與枯草芽孢桿菌的共培養(yǎng)及其對活性次生代謝產(chǎn)物的影響
更多全文請查看鏈接：http://journals.im.ac.cn
聲明：本文由《微生物學(xué)報》授權(quán)生物谷 www.bioon.com 網(wǎng)站發(fā)布，如需轉(zhuǎn)載請直接與中國科學(xué)院微生物研究所期刊聯(lián)合編輯部聯(lián)系并支付相應(yīng)費用,，未經(jīng)授權(quán)不得轉(zhuǎn)載,，若轉(zhuǎn)載將付相應(yīng)的法律責(zé)任。
