微生物學(xué)通報(bào) OCT 20,2010,37(10):1558～1565
工業(yè)腐敗微生物種屬特性及抗藥性分析
陳藝彩 謝小保 施慶珊 歐陽友生* 陳儀本
(廣東省微生物研究所 廣東省菌種保藏與應(yīng)用重點(diǎn)實(shí)驗(yàn)室 廣東省微生物應(yīng)用新技術(shù)公共實(shí)驗(yàn)室 廣東 廣州 510070)
摘 要: 為科學(xué)治理工業(yè)領(lǐng)域中微生物對殺菌劑的抗藥性, 從工業(yè)產(chǎn)品、原料及水樣中采集腐敗微生物, 細(xì)菌按照《常見細(xì)菌系統(tǒng)鑒定手冊》,、API鑒定系統(tǒng)及16S rDNA序列分析, 真菌按《真菌鑒定手冊》及18S rDNA序列分析分別進(jìn)行鑒定; 通過測定殺菌劑的最小抑制濃度(MIC)來評估微生物抗藥性水平,。結(jié)果顯示, 腐敗微生物中革蘭氏陰性細(xì)菌約占46.91%, 主要包括假單胞菌屬、腸桿菌屬,、氣單胞菌屬、克雷伯氏菌屬等; 革蘭氏陽性菌約占32.71%, 主要種屬為芽孢桿菌屬,、微桿菌屬,、李斯特氏菌屬及球菌等; 真菌約占12%, 主要包括青霉屬、木霉屬和曲霉屬,。MIC測試結(jié)果顯示, 主要抗藥性微生物為假單胞菌屬, 約占33.78%, 平均抗性水平達(dá)到36 mg/L, 且傳代不穩(wěn)定,。結(jié)論認(rèn)為, 工業(yè)上微生物污染主要由細(xì)菌耐藥性引起, 細(xì)胞膜結(jié)構(gòu)及細(xì)菌生物膜的形成在該類殺菌劑抗藥性產(chǎn)生的過程中起重要作用。
關(guān)鍵詞: 微生物抗藥性, 工業(yè)殺菌劑, 最小抑制濃度
Species Identification of Industry Spoilage Microorganism and the Resistance Analysis
CHEN Yi-Cai XIE Xiao-Bao SHI Qing-Shan OUYANG You-Sheng* CHEN Yi-Ben
(Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangzhou, Guangdong 510070, China)
Abstract: In the aim of preventive microorganism resistance to industrial biocides, a collection of micro-organism which isolated from industry production and raw material were gathered. To characterize bacte-ria species according to Manual of Systematic and Determinative Bacteriology, API Identification System, 16S rDNA genes analysis, and fungus according to Manual for the Identificati of Fungi, 18S rDNA genes analysis, respectively. The minimum inhibitory concentrations for biocides were determined to estimate the microorganisms resistance. It was found that the majority of isolates in the collection were Gram-negative bacteria (46.91%) including genera Pseudomonas, Enterobacter, Aeromonas, Klebsiella and Burkholderia. The dominant strains of Gram-positive bacteria (32.71%) were genera Bacillus, Mi-crobacterium, Arthrobacter, Listeria along with several coccus. And fungi (12%) were Aspergillus, Trichoderma and Penicillium. The value of MICs showed that main resistant species is Pseudomonas (33.78%), its average level of resistance was up to 36 mg/L and the resistance of stability was weak with subculture. The result indicated that one of the considerable reasons of biological pollution in in-dustry was the microorganism tolerance and the nature of the membrane structure and the biofilm for-mation of bacteria probably played a important role in the formation of microorganism resistance.
Keywords: Microorganism resistance, Biocide, Minimum inhibitory concentration
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