雙酚A（BPA）使用量大,，在環(huán)境中存在廣泛,，是備受關(guān)注的內(nèi)分泌干擾物之一。中國科學(xué)院城市環(huán)境研究所城市環(huán)境與健康重點(diǎn)實(shí)驗(yàn)室環(huán)境生物技術(shù)研究組致力于新型污染物遷移轉(zhuǎn)化的研究,，近期研究揭示了氨氧化細(xì)菌對(duì)BPA的轉(zhuǎn)化機(jī)制,。
該研究發(fā)現(xiàn)，氨氧化細(xì)菌可將氨氮轉(zhuǎn)化為亞硝氮,，而后通過非生物的硝化作用（abiotic nitration）,，BPA會(huì)被轉(zhuǎn)化為硝基取代BPA（nitro-BPA、dinitro-BPA）,。對(duì)實(shí)際樣品的研究發(fā)現(xiàn),，污水廠的出水中存在硝基取代BPA，表明BPA硝化作用是污水廠中BPA轉(zhuǎn)化的途徑之一,。
本研究結(jié)果為深入了解氨氧化細(xì)菌去除內(nèi)分泌干擾物提供了理論依據(jù),。
該研究以助理研究員孫倩博士為第一作者，研究員于昌平博士為通訊作者,。該工作得到中國科學(xué)院,、科技部和廈門市的資助,。相關(guān)成果“Transformation of Bisphenol A and Alkylphenols by Ammonia-Oxidizing Bacteria through Nitration，”已在環(huán)境研究期刊Environmental Science & Technology在線發(fā)表,。（生物谷Bioon.com）
doi:10.1021/es204424t
PMC:
PMID:
Transformation of Bisphenol A and Alkylphenols by Ammonia-Oxidizing Bacteria through Nitration
Qian Sun†, Yan Li†, Pei-Hsin Chou‡, Po-Yi Peng‡, and Chang-Ping Yu*†
Transformation of bisphenol A (BPA) by ammonia-oxidizing bacteria (AOB) Nitrosomonas europaea ATCC 19718 was investigated. On the basis of the ultraperformance liquid chromatography (UPLC) coupled to quadrupole time-of-flight mass spectrometry (Q-TOF MS) and nuclear magnetic resonance analysis, we found N. europaea could transform BPA into nitro- and dinitro-BPA, suggesting that abiotic nitration between the biogenic nitrite and BPA played a major role in the transformation of BPA in the batch AOB system. Nitrite concentrations, temperature, and pH values were the major factors to influence the reaction rate. Furthermore, the yeast estrogenic screening assay showed that the formed nitro- and dinitro-BPA had much less estrogenic activity as compared with its parent compound BPA. Similar reactions of abiotic nitration were considered for 4-n-nonylphenol (nNP) and 4-n-octylphenol (nOP) since nitro-nNP and nitro-nOP were detected by UPLC-Q-TOF MS. In addition, results from the local wastewater treatment plant (WWTP) showed the occurrence of nitro-BPA and dinitro-BPA during the biological treatment process and in the effluent, indicating that nitration of BPA is also a pathway for removal of BPA. Results of this study provided implication that AOB in the WWTPs might contribute to removal of selected endocrine-disrupting compounds (EDCs) through abiotic nitritation.
