日本埼玉醫(yī)科大學(xué)細菌學(xué)教授三谷幸之介為首的科研小組日前開發(fā)了有效操作胚胎干細胞(ES細胞)基因的技術(shù),，該成果被刊登在8月25日發(fā)行的美國《國家科學(xué)院院刊》（PNAS）上,。
胚胎干細胞是一種可成長為任何人體器官組織的“萬能細胞”,。
據(jù)日本共同社報道,，該科研小組稱,，人類ES細胞基因操作在技術(shù)上一直存在困難,。據(jù)悉,，若使用新開發(fā)的技術(shù)方法,，科研人員可將疾病的基因植入ES細胞,，從而為制成再現(xiàn)癥狀的細胞提供方便，可能將加快有效治療藥物的研究,。
科研小組使用了三谷教授開發(fā)的低毒性改良型病毒媒介作為將基因植入細胞的攜帶媒介,。實際實驗中將基因植入人類ES細胞后，目標位置的基因植入率為45%左右,，比以往的1%有大幅提高,。據(jù)悉，病毒原本攜帶的基因已事先被除去,，因此也不存在安全性的問題,。
據(jù)三谷教授稱，該技術(shù)也將有助于新型萬能細胞“iPS細胞”的研究,。（生物谷Bioon.com）
生物谷推薦原始出處：
PNAS published August 25, 2008, doi:10.1073/pnas.0806136105
Heparin promotes the growth of human embryonic stem cells in a defined serum-free medium
Miho K. Furue*,?,?, Jie Na?, Jamie P. Jackson?, Tetsuji Okamoto§, Mark Jones?, Duncan Baker?, Ryu-Ichiro Hata‖, Harry D. Moore?, J. Denry Sato**, and Peter W. Andrews
A major limitation in developing applications for the use of human embryonic stem cells (HESCs) is our lack of knowledge of their responses to specific cues that control self-renewal, differentiation, and lineage selection. HESCs are most commonly maintained on inactivated mouse embryonic fibroblast feeders in medium supplemented with FCS, or proprietary replacements such as knockout serum-replacement together with FGF-2. These undefined culture conditions hamper analysis of the mechanisms that control HESC behavior. We have now developed a defined serum-free medium, hESF9, for the culture of HESCs on a type I-collagen substrate without feeders. In contrast to other reported media for the culture of HESCs, this medium has a lower osmolarity (292 mosmol/liter), l-ascorbic acid-2-phosphate (0.1 μg/ml), and heparin. Insulin, transferrin, albumin conjugated with oleic acid, and FGF-2 (10 ng/ml) were the only protein components. Further, we found that HESCs would proliferate in the absence of exogenous FGF-2 if heparin was also present. However, their growth was enhanced by the addition of FGF-2 up to 10 ng/ml although higher concentrations were deleterious in the presence of heparin.
