據(jù)9月25日的《科學(xué)》（Science）雜志報道說，應(yīng)用不會自身被整合到宿主基因組內(nèi)的病毒,，科學(xué)家已經(jīng)研發(fā)出了一種能產(chǎn)生“誘導(dǎo)全能干細胞”（iPS 細胞）的方法,，因而繞過了使得以往iPS細胞的成功遭到破壞的一個經(jīng)常遇到的問題,。 過去，科學(xué)家們已經(jīng)培育出了類似胚胎干細胞的iPS細胞,，他們所用的方法包括通過可能有害的病毒將特別的DNA-結(jié)合蛋白導(dǎo)入到細胞中,，而這種做法常常改變了這些細胞的基因組并引起動物中的腫瘤。這一新的發(fā)現(xiàn)證明,，人們可以培育出沒有永久性基因損害的iPS,，而這種以往的基因損害與人們的設(shè)計有關(guān)。這一發(fā)現(xiàn)代表了人們在未來臨床上應(yīng)用iPS細胞邁出了重大的一步,。
Matthias Stadtfeld及其同事培育出了他們的iPS細胞（這些細胞具有成長為多種其它如肺,、腦及心臟細胞等特別細胞的潛能），他們說,，他們還沒有觀察到這些細胞有任何有害的副作用,。過去，類似的重新編程細胞顯示,，它們可以減輕小鼠模型中巴金森氏病和鐮刀樣貧血的癥狀,。因此這一新的發(fā)現(xiàn)可能也會導(dǎo)致對人類疾病的細胞療法的進展。但是,，研究人員說,，重要的是,，人們需要測定用這種方法生產(chǎn)的人類iPS細胞在可能的臨床應(yīng)用中是否和人類胚胎干細胞一樣有效。（生物谷Bioon.com）
生物谷推薦原始出處：
Science,，DOI: 10.1126/science.1162494,，Matthias Stadtfeld，Konrad Hochedlinger
Induced Pluripotent Stem Cells Generated Without Viral Integration
Matthias Stadtfeld 1, Masaki Nagaya 2, Jochen Utikal 1, Gordon Weir 2, Konrad Hochedlinger 1*
1 Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA.; Harvard Stem Cell Institute, Cambridge, MA 02138, USA.; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.; Department of Medicine, Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA.
2 Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, One Joslin Place, Boston, MA 02115, USA.; Department of Medicine, Harvard Medical School, 25 Shattuck Street, Boston, MA 02115, USA.
Pluripotent stem cells have been generated from mouse and human somatic cells by viral expression of the transcription factors Oct4, Sox2, Klf4, and c-Myc. A major limitation of this technology is the use of potentially harmful genome-integrating viruses. Here, we generate mouse induced pluripotent stem cells (iPS) from fibroblasts and liver cells by using nonintegrating adenoviruses transiently expressing Oct4, Sox2, Klf4, and c-Myc. These adenoviral iPS (adeno-iPS) cells show DNA demethylation characteristic of reprogrammed cells, express endogenous pluripotency genes, form teratomas, and contribute to multiple tissues, including the germ line, in chimeric mice. Our results provide strong evidence that insertional mutagenesis is not required for in vitro reprogramming. Adenoviralreprogramming may provide an improved method for generating and studying patient-specific stem cells and for comparing embryonic stem cells and iPS cells.
